水稻白化突变体alb24的遗传分析及其基因定位

Genetic Analysis and Gene Mapping of an Albino Mutant in Rice( Oryza sativa L. )

[目的]对水稻叶色发育缺陷突变体的研究有助于阐释高等植物叶绿体发育和光合作用所必需的基因网络。[方法]从籼稻品种华粳籼74发展的一个株系中出现幼苗期叶片白化、四叶期枯死的表型分离,通过与粳稻中花11构建F2群体和分子标记,对突变体进行遗传分析和基因定位。[结果]该白化突变体由一对隐性核基因控制,暂命名为alb24,定位于第6染色体SSR标记RM276和Indel标记ID4955-1之间,遗传距离分别为5.0和0.1 cM。[结论]ALB24基因的初步定位为进一步的精细定位和克隆该基因奠定了基础。

[ Objective ] To study on defective mutants of rice leaf development helps explain chloroplast development and gene networks required for photosynthesis in higher plants. [ Method ] A mutant line developed from an irutica variety Huajingxian74 showed segragation of albino leaves in seedling stage and lethality in four-leaf stage. Genetic analysis and gene mapping of the mutant was conducted through the development of mo- lecular markers and F2 population derived from the cross between a japonica variety Zhonghua 11 and the mutant. [ Result] The genetic analysis showed that the albino phenotype was controlled by a single recessive gene, named as alb24, and ALB24 was mapped between SSR maker RM276 and InDel maker ID4955-1 on chromosome 6, with genetic distances of 5.0 and 0.1 cM, respectively. [ Conclusion] The results of primary map- ping of ALB24 laid the foundation for further fine mapping and cloning of the gene.