球面对称设计优化毛蕊老鹳草总黄酮的提取工艺

Optimization of Extraction Technology for Total Flavonoids in Geranium eriostemon FISch. ex DC. by Spherical Symmetric Design

[目的]采用球面对称设计优化毛蕊老鹳草总黄酮的醇提取工艺。[方法]以提取时间、乙醇浓度、料液比为自变量,总黄酮得率为因变量,对自变量各水平进行多元回归和二项式拟合,通过球面对称设计法优选提取工艺条件,并进行预测分析。[结果]最佳提取条件为:料液比1∶40(g/ml),乙醇浓度60%,提取时间35 min;在此优条件下,总黄酮的理论得率为1.91%。[结论]球面对称设计法优选毛蕊老鹳草总黄酮提取工艺,方法简便,预测性良好。

[ Objective ] To optimize extraction technology of total flavonoids in Geranium eriostemon FISch. ex DC. by spherical symmetric de- sign. [ Method] With extraction time, solid-liquid ratio, alcohol volume fraction as independent variables and flavonoids yield as the dependent variable, no-liner mathematic model was used to estimate the relationship between independent variables and dependent variable, spherical sym- metric design was used to optimize extraction technology, and prediction was carried out through comparing the observed and predicted value. [ Result ] The analysis indicated the optimum conditions were 40-fold solvent, 60% ethanol and 35 min for extraction. [ Conclusion ] Spherical symmetric design was convenient and highly predictive for optimizing extraction technology of total flavonoids.