成都平原主养草鱼·搭配鲫鱼池塘底泥中的细菌群落结构分析

Investigation of Bacterial Community Structure in Chengdu Fish- farm Sediment Stocked with Dominated Grass Carp and Subordinate Crucian

[目的]分析成都平原主养草鱼、搭配鲫鱼池塘底泥中的细菌群落结构。[方法]通过构建细菌16S rRNA基因克隆文库,对阳性克隆子进行多样性分析和构建系统发育树。[结果]依据97%序列相似性划分OUT,所构建的文库共获得171个克隆子,并划分为14个OTUs(操作分类单元),其多样性指数Shannon(H)和优势度指数Simpson(D)分别为2.12和7.15,丰富度指数SChao1和SACE均为16。池塘底泥中主要的细菌类群为:梭杆菌门(24%)、变形菌门(55%)、浮霉菌门(10.5%)、绿弯菌门(5.9%)、拟杆菌门(2.3%)、放线菌门(1.8%)和厚壁菌门(0.6%)。[结论]Cetobacterium、Prolixibacter、Rhodocyclus、Candidate、Planctomycetacia、Thiocapsa、Desulfomicrobium和Sterolibacterium等属菌在脱氮除磷、降低硫化物、降解有机质等发挥重要作用。池塘底泥既是水生态系统修复功能菌的资源库,但也存在着Xanthomonas等致病性和功能未知的细菌库。

[ Objective ] The paper was to investigate bacterial community structure in Chengdu fish-farm sediment stocked with dominated Grass Carp and subordinate Crucian. [ Method] Through the construction of 16S rRNA cloning library of bacteria, the positive clone was carried out community diversity analysis, and the phylogenetic tree was constructed. [ Result] OUT （Operational Taxonomic Units） was classified according to sequences similarity of 97%, all 171 clones were clustered into 13 OTUs. The richness index Schao1 and SAcE were 16 and 16, and the dominancy index （D） and diversity index （H） were 2.12 and 7.15, respectively. All sequences were respectively fell into the phylum of Fusobacteria （24%）, Proteobacteria （55%）, Planctomycetes （ 10.5% ） , Chloroflexi （5.9%） , Bacteroidetes （2.3%）, Actinobacteria （ 1.8% ） and Firmicutes （0.6%）. [ Conclusion] The members of Cetobacterium, Prolixibacter, Rhodocyclus, Candidate, Planctomycetacia, Thiocapsa, Des ulfomi- crobium and Sterolibacterium play a key role in denitrogenation, phosphorus and sulfate reducing and organics degradation, while the Xan- thomonas was an plant pathogen, this result suggested that the sediment was not only a bacterial source for ecological restoration, but also a reservoir for pathogenicity.