桂枝提取物抑菌活性研究

Study on antifungal activity of Ramulus cinnamomi

以柑橘青、绿霉菌及猕猴桃灰霉菌等17种植物病原菌为指示菌，分别采用杯碟法、菌丝生长速率法对桂枝提取物的抑菌活性进行研究，结果表明桂枝提取物具有较广泛的抑菌谱，对这17种供试病原菌均表现出一定的抑制效果，其中对柑橘青、绿霉的抑菌图大小分别为（32．67±0．23）mm和（30．50±0．17）mm，最小抑菌浓度（MIC）分别为3．125mg／mL和6．25mg／mL，最小杀菌浓度（MFC）分别为6．25mg／mL和12．5mg／mL，而在其他15种植物病原菌中，桂枝提取物对车前草穗枯菌的活性最强，半抑制浓度（EC_50）为0．4386mg／mL，对其他病原菌的EC。为1～22mg／mL。

Seventeen kinds of phytopathogens such as Penicillium italicum, Penicillium digitatum and Botrytis cinerea were used as indicators to explore the antifungal activities of Ramulus cinnamomi by cyiind-plate and mycelial growth rates methods,respectively. The results showed that Ramulus cinnamomi had a broader spectrum antifungal activity against the 17 sorts of tested phytopathogens ,the inhibition zones against P.italicum and P.digitaturn were （32.67±0.23）mm and （30.50±0.17）mm,respectively. While the minimum inhibitory concentrations（MIC） gainst P.italicum and P.digitatum were 3.125mg/mL and 6.25mg/mL,respectively, and the minimum fungicidal （MFC） concentrations were 6.25mg/mL and 12.5mg/mL, respectively. Meanwhile, the toxicity of indoor test of Ramulus cinnamomi against the other 15 phytopathogens showed that Ramulus cinnamomi extracts had the best antifungal activity on Diaporthe angeficae with the ECso value of 0.4386mg/mL. The antifungal activities against other tested pathogens with ECso values ranged from 1 to 22mg/mL.