DADS对人肝癌细胞株HepG_2增殖和凋亡的影响

Effect of diallyl disulfide on the growth inhibition and apoptosis in human HepG_2 cancer cell line in vitro

目的:探讨二烯丙基二硫(DADS)对人肝癌细胞株HepG2增殖和凋亡的影响。方法:采用MTT、细胞计数检测DADS对人肝癌细胞株HepG2增殖的影响;应用流式细胞仪检测DADS对HepG2细胞周期和细胞凋亡率的影响。结果:DADS使细胞增殖明显受抑制且抑制率呈药物浓度时间依赖性(P<0.05),DADS组细胞的倍增时间延长(P<0.05)。与对照组比较DADS处理组G2/M期细胞比例显著增加,而G0/G1期细胞比例则明显下降,细胞的凋亡率显著增加(P<0.05)。结论:DADS对人肝癌细胞株具有抗增殖作用,且与药物浓度及作用时间相关。DADS能阻滞细胞周期在G2/M期,并诱导细胞凋亡。

Objective： To investigate the effect of diallyl disulfide （DADS） on the growth inhibition and apoptosis in human HepG2 cancer cell line in vitro. Methods： Effect of DADS on HepG2 Cancer cell line proliferation was measured by MTT assay and cell counts. The apoptosis rate and phase distribution of cell cycle of cell line induced by DADS was detected by using flow cytometry （FCM） with PI staining. Results： DADS obviously inhibited cells proliferation, and the inhibition rate assumes the dependence of the drug concentration and time （P〈0.05） ; cell doubling time in DADS group extended （P〈0.05）. Compared with the control group, G2/M phase cell fraction increased significantly in the DADS treatment group, while G0/G1 phase cell fraction decreased obviously,and the cell apoptosis rate increased significantly （P〈0.05）. Conclusions： DADS could significantly inhibit the proliferation of human HepG2 cancer cell line and this effect presents dose-dependent and time-dependent. Besides, it can inhibit the cell cycle in G2/M phase and induce apoptosis.