大鼠心肌梗死后血清脑钠肽、内皮素1和一氧化氮的变化及相关性研究

SERUM LEVELS OF BRAIN NATRIURETIC PEPTIDE,ENDOTHELIN-1 AND NITROGEN MONOXIDE AFTER ACUTE MYOCARDIAL INFARCTION AND CORRELATION IN RATS

目的观察急性心肌梗死(acute myocardial infarction,AMI)后大鼠脑钠肽(brain natriuretic peptide,BNP)、内皮索1(endothelin-1,ET-1)和一氧化氮(nitrogen monoxide,NO)的变化以及相关性。方法 Wistar大鼠80只,经麻醉后行冠状动脉结扎处理制作心肌梗死模型,术后存活的大鼠分为对照组(Sham,n=40)和心肌梗死组(AMI,n=40)。术后开始计时,分别于6h、12h、24h、3d、7d和30d抽取大鼠静脉血1mL,测定BNP、ET-1和NO水平,并分析三者的相关性。结果 2组BNP、ET-1和NO水平在组间、不同时点以及组间和不同时点的交互作用差异均有统计学意义(P<0.01);相关性分析提示ET-1与NO呈负相关(r=-0.948,P=0.004)。结论 AMI后出现了血清BNP、ET-1升高和NO降低的变化。为AMI后神经内分泌激素的变化提供了理论依据。

Objective To explore serum levels of brain natriuretic peptide （BNP） ,endothelin-1 （ET-1） and nitrogen monoxide（NO） in rats after acute myocardial infarction （AMI） and to observe their correlation. Methods Eighty male Wister rats were randomly divided into two groups ： sham group （ n = 40）, AMI group （n = 40 ）. The serum concentrations of BNP, ET-1 and NO were measured at different time （6h, 12h,24h,3d,7d and 30d） to compare the difference between two groups, and their correlation were analyzed. Results There were significant difference in the serum levels of BNP, ET-1 and NO between AMI group and sham group （P 〈 0.01 ）. For different groups, there were significant differences in the serum levels of BNP,ET-1 and NO among different time （P 〈0.01 ）. From the interactional effort of group and time, there were different evolvement in the serum levels of BNP, ET-1 and NO between two groups （P 〈0.01 ）. The analysis in AMI group showed that serum ET-1 was negatively correlated with serum NO. Conclusion This study showed serum BNP and ET-1 increase and serum NO decrease after AMI, which may lay theory evidence for neural endocrine hormone change.