玉米花粉多糖对猪瘟免疫效果的研究

Study on effect of corn pollen polysaccharides on immunity of pig

本研究采用醇沉淀法从 350 g玉米花粉提取得粗提复合物 38.3 g。经用蒽酮反应、α萘酚反应、间苯二酚反应、碘化反应及高效液相色谱法鉴定 ,证实为多糖。用上述多糖作为免疫增强剂按剂量 50、 1 0 0、2 0 0 mg分别与猪瘟弱毒细胞冻干苗共同免疫猪 ,测定其免疫效果。结果表明 ,各剂量组的 IHA抗体效价均高于对照组 ;T细胞活性 E花环试验测得各剂量的活性 E花环百分率分别为 2 1 .84 %、2 5.4 6%、2 1 .92 % ,均高于对照组 1 5.91 % ;T淋巴细胞转化试验显示 ,各试验组的 T淋巴细胞转化率分别为 55.32 %、 56.39%、54.2 7% ,均高于对照组 4 7% ,上述三种方法测定的结果均显示显著差异。对 T淋巴细胞亚群的测定结果为 ,各试验组的 CD3、CD4、CD8T淋巴细胞的测定平均值均明显高于对照组 ,但 NK细胞的变化不显著。根据试验结果选取 1 0 0 mg剂量组在两个猪场进行田间扩大试验 ,测得的 IHA抗体效价亦高于对照组。研究结果表明 ,玉米花粉多糖既可增强体液免疫 ,又能增强细胞免疫。

In this study we had obtainded 38.8g of crude extract from cornpollen using alcohol precipitation method. The crude extract was identified as polys accharides after detection by anthraone,molish, seliwanoff, iodide reactions and high-performance liquid-phase chromatograph measure. The corn pollen polysaccharides(CPP) was used as immunol ogical enhancer and its effect was detected by using 50, 100 and 200 mg of CPP m ixing with hog cholera lapinized virus (HCLV). As result, IHA antibody titers of three groups using CPP were higher than that of control. In the T lymphocyte ac tive-Erosette test, it showed average rate 21.84%, 25.46% and 21.92% of E-rosette in three groups and all groups were higher than 15.91% of control. The Tlymp hocyte transformation rate of three experimental groups were 55.32%, 56.39% and 54.27 % ,respectively, and three groups were higher than 47% of control. The results of three methods demonstrated that CPP could promote immune effect when it was mi xed with HCLV vaccine. T lymphocyte subgroup assay was also carried out by count ing CD3, CD4 and CD8.T lymphocyte and their average value were higher than that of control. However, NK cells of three experimental groups did not show signific antly changes contrast with that of control. According to results above,100 mg CPP was selected to do field trial, and the IHA antibody level was also showed hig her than that of control. The results in this experiment demonstrated that CPP could be used as an immunological enhancer to promote humoral and cellular-immunity.