小麦麸皮总黄酮清除DPPH自由基活性的研究

Study on the Scavenging Effect of Wheat Bran Total Flavones on 2,2-Diphenyl-1-Picrylhydrazyl Radical

以小麦麸皮为原料,乙醇为提取溶剂,超声波辅助提取总黄酮。考察了12种大孔吸附树脂对没食子酸的吸附量,选用LSA-21大孔吸附树脂对小麦麸皮总黄酮进行吸附,60%乙醇溶液解吸。分别考察了不同浓度的Vc、小麦麸皮总黄酮粗提物和纯化物对1,1-二苯基-2-苦苯肼自由基(DPPH·)的清除效果。结果表明:LSA-21大孔吸附树脂对没食子酸的吸附能力较强,吸附量达0.21 mg/g。Vc、小麦麸皮总黄酮粗提物和纯化物对DPPH·均有清除作用,其清除能力大小顺序为:小麦麸皮总黄酮纯化物>小麦麸皮总黄酮粗提物>Vc。

Ultrasonic method was used to extract the total flavones from wheat bran, and alcohol-water was used as solvent. Twelve kinds of different macroporous were studied to adsorb polyphenols, the macroporou of LSA-21 was chosen, then ethanol solution of 60% was used for desorption. The scavenging ability of Vc, total flavones of crude extracts and the purified compound on DPPH· were studied. The research shows that the capacity of macroporous＇ adsorption was stronger, and the adsorption was the highest as 0.21 mg/g. Vc, total flavones of crude extracts and the purified compound have effect on DPPH radical scavenging and the sequence of free radical scavenging activity is total flavones of crude extracts 〉 total flavones of the purified compound 〉 Vc.