桂黄降脂颗粒质量标准研究

Study on The Quality Standard of Guihuang Jiangzhi Grain

目的：建立桂黄降脂颗粒的质量标准。方法：采用薄层色谱法对桂黄降脂颗粒中山楂、丹参进行定性鉴别；采用HPLC法对山楂中的槲皮素和丹参中的丹参素钠进行含量测定。结果：薄层色谱斑点清晰、分离度效果较好、且阴性无干扰；槲皮素在11.2～33．6μg范围内呈良好的线性关系，r=0．9995（n=5），样品平均回收率为98．00％，RSD1．33％；丹参素钠在0．0166-0．332mg·mL^-1。范围内呈良好的线性关系，r=0．9998（n=5），样品平均回收率为97．87％，RSD1．35％。结论：该方法准确可靠、专属性强、重现性好，可以作为控制桂黄降脂颗粒质量的有效方法。

Objective： To establish the quality standard of Guihuang Jiangzhi Grain. Methods： TLC method was used to identify Hawthor, Salvia miltiorrhiza ;HPLC method was used to quantitatively analyze the concentration of quercetin and sodium danshensu in the Grain. Results： The TLC spots were clear without interference of the negative control. The linear range of quercetin was 11.2-33.6 μg, r=0.9995（n=5）, with the average recovery of 98.00% RSD 1.33%. The linear range of sodium danshensu was 0.0166-0.332 mg. mL^-1, r=0.9998 （n=5）, with the average recovery of 97.87% RSD 1.35%. Conclusion： The method is accurate, reliable, specific and reproducible, and it can be used for the quality control of Guihuang Jiangzhi Grain.