摘要
目的:建立桂黄降脂颗粒的质量标准。方法:采用薄层色谱法对桂黄降脂颗粒中山楂、丹参进行定性鉴别;采用HPLC法对山楂中的槲皮素和丹参中的丹参素钠进行含量测定。结果:薄层色谱斑点清晰、分离度效果较好、且阴性无干扰;槲皮素在11.2~33.6μg范围内呈良好的线性关系,r=0.9995(n=5),样品平均回收率为98.00%,RSD1.33%;丹参素钠在0.0166-0.332mg·mL^-1。范围内呈良好的线性关系,r=0.9998(n=5),样品平均回收率为97.87%,RSD1.35%。结论:该方法准确可靠、专属性强、重现性好,可以作为控制桂黄降脂颗粒质量的有效方法。
Objective: To establish the quality standard of Guihuang Jiangzhi Grain. Methods: TLC method was used to identify Hawthor, Salvia miltiorrhiza ;HPLC method was used to quantitatively analyze the concentration of quercetin and sodium danshensu in the Grain. Results: The TLC spots were clear without interference of the negative control. The linear range of quercetin was 11.2-33.6 μg, r=0.9995(n=5), with the average recovery of 98.00% RSD 1.33%. The linear range of sodium danshensu was 0.0166-0.332 mg. mL^-1, r=0.9998 (n=5), with the average recovery of 97.87% RSD 1.35%. Conclusion: The method is accurate, reliable, specific and reproducible, and it can be used for the quality control of Guihuang Jiangzhi Grain.
出处
《现代生物医学进展》
CAS
2014年第15期2952-2955,2961,共5页
Progress in Modern Biomedicine
基金
四川省泸州市科技局中医药研究基金项目(20110315)