PXD101对口腔鳞癌HN-6细胞增殖及凋亡的初步研究

Research on the Apoptosis of Oral Squamous Cell Carcinoma HN-6 Induced by Histone Deacetylase Inhibitor PXD101

目的:本实验探讨组蛋白去乙酰化酶抑制剂PXD101对口腔鳞癌HN-6细胞的增殖、细胞凋亡及细胞周期的影响。方法:PXD101对口腔鳞癌HN-6细胞进行干预,倒置相差显微镜观察细胞形态学改变;MTT法检测PXD101对HN-6细胞的增殖影响;Annexin-V-FITC/PI双染流式细胞仪定量检测细胞凋亡;流式细胞仪分析细胞周期。结果:PXD101可明显抑制HN-6细胞的生长(P<0.05),呈时间剂量依赖性。倒置相差显微镜下观察对照组细胞贴壁,形态呈多边形,生长活跃;实验组细胞脱壁,变小,细胞核皱缩。绘制细胞生长曲线示,随着PXD101的浓度和作用时间的增加,HN-6细胞生长明显受到抑制,各实验组细胞生长抑制率与对照组比较,P<0.05差别有统计学意义。1μmol/LPXD101作用24 h,48 h细胞总凋亡率分别为20.9%、38.6%,与对照组相比有统计学意义(P<0.05);HN-6细胞周期阻滞于G0/G1期,与对照组相比,P<0.05差别有统计学意义。结论:PXD101体外实验能显著抑制人口腔舌癌HN-6的细胞增殖,诱导细胞凋亡。

Objective： This study was purposed to explore the effect of PXD101 on the cell proliferation, cycle arrest and apoptosis of oral squamous cell carcinoma （HN-6） in vitro. Methods： The oral squamous cell carcinoma HN-6 cells cultured in medium were intervened PXD101 and were observed by the inverted phase contrast microscope. The effect of PXD101 on Proliferative activity of HN-6 cells was detected by MTT method. The cell apoptotic rates were determined by flow cytometry through Annexin -V-FITC/PI staining.cell cycle arrest rates were analyzed by flow cytometry. Results： PXD101 can obviously inhibit the growth of HN-6 cells （P 〈0.05） and show time dose dependent. Under the inverted phase contrast microscope, Control cell morphology has not changed, with cells polygonal, adherent and active. However, the morphology of the cells treated by PXD101 has changed. The groups form a smaller and shrivel, cells with the increase of concentration and action time of PXD101, cell growth is restrained obviously, the growth inhibition of the cells compared with control group, P 〈0.05, differences have statistical significance. Cell apoptosis rate were 20.9%, 38.6%, respectively with 1 μmol/L PXD101treatment for 24 h, 48 h compared with the control group was statistically significant （P 〈0.05）. HN-6 block cell cycle in G0/G1 phase, compared with the control group, P 〈0.05, differences have statistical significance. Conclusion： PXD101 in vitro experiment can significantly inhibit people HN-6 oral squamous carcinoma cell proliferation and induce apoptosis.