柴半六合汤对慢性肾衰竭模型大鼠血清胃泌素、胃动素及生长抑素的影响(英文)

The Effect of Chaibanliuhe decoction on GAS,MTL and SS in Rat Models with Chronic Renal Failure

目的:观察柴半六合汤对慢性肾衰竭(CRF)模型大鼠血清胃泌素(GAS)、胃动素(MTL)、生长抑素(SS)水平的影响,探讨其作用机制。方法:采用5/6肾切除的方法复制CRF大鼠模型,设立假手术组、模型组、柴半六合汤高剂量组、低剂量组、尿毒清对照组,检测血肌酐(Scr)、尿素氮(BUN)、胃泌素(GAS)、胃动素(MTL)及生长抑素(SS),取胃粘膜行HE染色,光镜下观察其组织病理变化。结果:用药各组大鼠治疗后Scr、BUN低于模型组;柴半六合汤高剂量组大鼠Scr、BUN、GAS、MTL低于低剂量组、SS高于低剂量组;柴半六合汤高、低剂量组大鼠GAS、MTL低于尿毒清对照组、SS高于尿毒清对照组。结论:柴半六合汤通过降低CRF模型大鼠GAS、MTL水平、SS升高水平,改善胃肠道症状。

Objective： To observe the effect of Chaibanliuhe decoction on the levels of Gastric secrete element（GAS）, Gastric dy- namic element（MTL）, somatostatin（SS） in rat chronic renal failure（CRF） model, and to explore its mechanism. Methods： The model of CRF in rats were established by the method of 5/6 nephrectomy. Rats were randomly divided into control group, model control group, high Chaibanliuhe decoction group, low Chaibanliuhe decoction group and Niaoduqing control group. The levels of Scr, BUN, GAS, MTL and SS in serum were detected, and the pathological changes of Gastric mucosa were observed by hematoxylin-eosin （HE） staining. Results： Compared with model control group, Scr, BUN, GAS and MTL decreased while SS increased significantly in every administra- tion group. The levels of Scr, BUN, GAS and MTL in the high Chaibanliuhe decoction group were lower than that in the low Chaibanliuhe decoction group and Niaoduqing control group while the level of SS was higher. Compared with that in the Niaoduqing control group, GAS and MTL decreased while SS increased in the low Chaibanliuhe decoction group. There was no significant difference between low Chaibanliuhe decoction group and Niaoduqing control group with the levels of Scr and BUN. Conclusion： Chaibanliuhe decoction can improve the gastrointestinal symptoms of CRF in rat model by decreasing the levels of GAS and MTL and increasing the level of SS.