低分子硫酸软骨素在兔软骨修复过程中对IL-1β、TNF-α和TGF-β及钙磷的影响(英文)

Influence of Low Molecular Weight Chondroitin Sulfate on IL-1β, TNF-αand TGF-β, Calcium and Phosphate in the Process of Cartilage Reconstruction of Rabbit

目的:探讨低分子量硫酸软骨素(CS)在兔软骨修复过程中对于IL-1β、TNF-α和TGF-β及血液中钙磷含量的影响。方法:36只成年新西兰大白兔,随机分成6组,分别为对照组、模型组、低分子量CS低剂量组、低分子量CS高剂量组、高分子量CS低剂量组、高分子量CS高剂量组,每组6只。通过在实验兔股骨髁关节面部位,钻出直径3mm,深度3mm的缺孔,造成其关节软骨的缺损。术后次日给予药物进行灌胃,每日1次,5周后取材。采用酶联免疫法测定关节液中IL-1β、TNF-α和TGF-β的含量,同时用全自动生化分析仪及其配套的试剂盒来测定兔血清中钙,磷含量。结果:低分子量CS和高分子量CS都能够减少关节液中炎性因子的含量和增加TGF-β的含量,且与模型组相比具有统计学意义(P<0.05);同时低分子量CS与高分子量CS相比效果较好(P<0.05);而高低剂量之间无统计学意义(P>0.05)。与模型组相比,给予CS的组别,其血清中的钙磷含量相对较少(P<0.05),但都高于对照组。结论:高、低分子量的CS都可以增加TGF-β的含量,降低IL-1β、TNF-α和血清中钙、磷的含量。而对于软骨修复,这可能是通过对上述因子的影响,从而产生积极的作用。

Objective： To investigate the influence of low molecular weight chondroitin sulfate on IL-1β, TNF-α and TGF-β, calcium and phosphate in the process of cartilage reconstruction of rabbit. Methods： 36 New Zealand rabbits were randomly divided into 6 groups with 6 rabbits in each group： control group, model group, low-dose of low molecular weight CS group, high-dose of low molecular weight CS group, low-dose of high molecular weight CS group and high-dose of high molecular weight CS group. A defect, 3mm diameter, 3mm depth, was drilled at the condyles of femur of the hind legs by using an electric bone drill. After the next day for animal drug perfusion, 1 time a day. Drawn after 5 weeks. The expression of cytokines, such as IL-1β, TNF-α and TGF-β in synovial fluid was detected by ELISA, determining levels of calcium and phosphorus in serum of rabbit by automatic biochemical analyzer kit. Results： Low molecular weight and high molecular weight CS were able to reduce levels of inflammatory cytokines and increase levels of TGF-β in the joint fluid. And compared with that in the model group, there is statistical significance （P〈0.05）. While low molecular weight CS＇ effect were better than high molecular weight CS （P〈0.05）. However, there was no obvious difference between high and low dose （P〉0.05）. Compared with that in the model group, the groups of CS, which levels of calcium and phosphorus in the serum were relatively low （P〈0.05）, but their levels were higher than that in the control group. However, these indexes had no obvious difference between low molecular weight CS group and high molecular weight CS. Conclusion： High and low molecular weight CS can increase the levels of TGF-β and reduce the levels of IL-1β, TNF-α and calcium, phosphate in the serum. For cartilage reconstruction, the above factors may have positive effect.