miR-134在人垂体腺瘤组织中的表达及其意义(英文)

Expression and Significance of miR-134 in the Tissues of Hypophyseal Adenoma

目的:探讨垂体腺瘤患者miR-134的表达及意义,分析其表达水平与无功能垂体腺瘤(non-functioningpituitaryadenomas,NFPA)增殖侵袭能力的相关性。方法:选择2010年6月至2013年7月本院收集垂体腺瘤标本104例以及8例尸检正常腺垂体的临床资料。采用实时荧光定量PCR、免疫组织化学技术检测Ki-67、MEG3、miR-134等在NFPA组织中的表达水平,并分析数据。结果:miR-134在NFPA组织中表达水平显著低于正常腺垂体和其他类型垂体腺瘤(P<0.01);miR-134的表达水平与NFPA患者肿瘤侵袭性、肿瘤细胞Ki-67阳性率及发病年龄呈负相关(P<0.01)。结论:miR-134表达下调可能是NFPA肿瘤发生及肿瘤呈侵袭样生长的重要因素,miR-134可作为诊断和评估NFPA预后的参考指标。

Objective： To detect the expression of miR-134 in normal pituitary tissue and each pituitary adenomas subtype, and to analyze the correlation between its expression level and the proliferation and invasion of non-functioning pituitary adenoma （NFPA）. Methods： To collect 104 cases with pituitary adenoma specimens （10 for ACTH adenoma, 18 for GH adenoma, 10 for PRL adenoma, namely, 66 cases ofNFPA）and 8 cases of autopsy normal pituitary and clinically relevant information. To use experimental techniques such as immunohistochemistry, quantitative real-time fluorescence PCR （qRT-PCR） and so on to detect the expression level of Ki-67, MEG3 andmiR-134, etc in each specimen, and then analyze the date in combination with clinical data. Results： The expression level of miR-134 in NFPA is significantly lower than that of other types of pituitary adenomas and normal pituitary （P〈0.01） while there exists negative correlation between the expression level of miR-134 and NFPA patients＇ age, the positive rate of tumor cells Ki-67 as well as tumor invasion （P〈0.01）. Conclusion： The down-regulation of miR-134 may be one of the important causes for the ocurrence and growth ofNFPA tumors,.miR-134 is expected to be a new target for the treatment of NFPA.