天然活性成分作用靶点识别及确证方法的研究进展

Advance in Methods of Identifying and Verifying Targets of Natural Products

药物靶标的发现和验证是新药研发的关键环节,对新药创制具有源头创新意义。天然产物是新药创制的重要来源,识别其作用靶点不仅为临床预防治疗提供可能新策略,也为进一步阐释中草药及其复方的作用特点及分子机制提供参考依据。随着生命科学和信息学的发展,药物靶点的识别及确证方法不断涌现,生物信息学、网络药理学、蛋白质组学、亲和色谱、药物亲和稳定性、芯片技术、基因敲除技术、RNA干扰等技术的广泛应用,越来越多的天然活性成分的靶点得以识别和验证。因此,本文对近五年来天然活性成分作用靶点识别及确证方法做一简要综述,以供参考。

Drug target discovery and validation is a key point in the process of new drug research and development, which is of original innovation for drug discovery. Natural products are important sources of drug development. It will not only provide a new strategy for the clinical prevention and treatment, but also provide some references for further exploration of functional characteristics and molecular mechanism of Chinese herbal medicine to identify the targets of natural products. With the development of life sciences and informatics, various methods for the identification of drug targets are developed. More and more targets of natural targets have been identified with the application of bioinformatics, proteomics, affinity chromatography, network pharmacology, drug stability of affinity, chip technology, gene knockout and RNA interference etc. Therefore, in this article, we review the related advances for recent five years to give some reference.