细胞联合培养在血管构建中的作用

The Effect of Cells Co-culture for Vascularization

组织工程三大要素为种子细胞、支架材料和信号分子,干细胞因其多分化潜能成为热门的种子细胞。血管化问题是制约工程化组织应用于临床的问题之一。利用干细胞构建组织工程血管的手段之一是在分离培养得到足够的种子细胞后,通过生长因子、细胞外基质、外力作用、其他细胞等的调控实现内皮向分化。只有实现了成功的血管构建,工程化组织才能正常的发挥作用。近年来不少国内外专家学者通过细胞联合培养的方法,观察细胞间的相互作用对血管构建的影响,结果表明,细胞联合培养在血管的形成、存活、稳定方面起到了重要的作用,为组织工程血管化提供了有效的途径,本文就部分细胞联合培养在血管构建中的作用作一综述。

Tissue engineering comprises seed cells, scaffold material and information factors. Stem cells with multiple-direction- al differentiation potential have been widely studied. Using stem cells to construct tissue-engineering blood vessels is an important mean which can realize endothelial differentiation through growth factors, extracellular matrix, mechanics environment, other cells et al after isolating and culturing enough seed cells. The vascularization of tissue engineering is one of the key problem which restricts clinical application. Only achieve successful vascularization, can engineered tissue works normally. In recent years, many scholars have been using different techniques to improve the vascularization. The results showed that cells co-culturing plays a key role in the formation, surviving and stability of blood vessels. What＇s more, it improves an effective means for tissue engineering vascularization. This paper just reviews part of these publication.