核糖体蛋白激酶磷酸化程度与人乳头瘤病毒16感染情况的相关性分析

Correlation of p70 S6 kinase phosphorylation with human papillomavirus type 16 infection in cervical cancer

目的:检测人乳头瘤病毒16型(HPV16)感染的宫颈鳞癌组织中核糖体蛋白激酶S6K和S6的磷酸化水平,探讨HPV16感染在宫颈癌发生发展中的作用。方法:利用PCR方法将收集到的135例人子宫颈鳞癌样本分为HPV16感染阳性组和阴性组,用免疫组织化学方法检测磷酸化S6K(p-S6K)和磷酸化S6(p-S6)蛋白在病例样本中的表达水平。结果:HPV16感染的宫颈癌组织内,核糖体蛋白S6K和S6的磷酸化比率分别为46.5%和68.7%,分别高于未感染的宫颈癌组织(8.33%和30.6%),差异具有统计学意义(P均<0.05)。两种蛋白相关性分析,p-S6K与p-S6蛋白在HPV16型病毒感染的宫颈癌组织中表达呈正相关性(r=0.095,P=0.019)。结论:HPV16病毒的感染与核糖体蛋白激酶S6K和S6的磷酸化(活化)具有强相关性,后两者的活化能促进细胞的生长增殖,在肿瘤的发生发展中起重要作用。

Objective： To study the expression and function of S6 kinase and S6 phosphorylation with human papillomavirus type 16 （HPV16） infection in cervical cancer. Methods： A total of 135 cervical cancer specimens were collected and detected HPV16 infection by polymerase chain reaction （PCR）, and analyzed the phosphorylation of S6 kinase and S6 protein expression level using immunohistochemistry method. Results： In the HPV16-infected cervical cancer speci- mens, the phosphorylation rates of S6 kinase （p-S6K） and S6 （p-S6） were 46.5% and 68.7%, while in the HPV16 negative specimens were 8.33% and 30.6%. The differences were statistically significant （P 〈 0.05）. There was a positive correlation of protein expression between p-S6K and p-S6 in the HPV16 infected cancer specimens （r=0.095, P =0.019）. Conclusion： HPV16 infection is strongly associated with the phosphorylation of S6 kinase and S6. S6 kinase and S6 phosphorylation promote cell proliferation, which play an important role in the pathogenesis and the development of cervical cancer.