酵母提取物诱导欧洲花楸悬浮细胞合成次生代谢产物的机制探究

Mechanism exploration on synthesis of secondary metabolites in Sorbus aucuparia cell cultures treated with yeast extract

以欧洲花楸悬浮培养细胞(SASC)为材料,检测酵母提取物(YE)处理后SASC生理生化指标的变化,初步探究YE诱导SASC合成次生代谢产物的机制。结果表明:YE诱导SASC合成了5种联苯类化合物,且5种化合物含量随诱导时间的延长呈现不同的变化规律;YE处理抑制细胞的生长;细胞培养液中的pH随着处理时间的延长逐渐降低;细胞中的可溶性蛋白质含量表现为缓慢下降的变化趋势,YE处理组(YE组)可溶性蛋白质含量与对照组(CK组)相比显著增加,最高时相对增量达到了147.76%;CK组和YE组细胞外Ca2+都表现为内流,但YE组的内流明显小于CK组。说明YE诱导使细胞处于一种胁迫状态,不利于细胞的生长,迫使细胞合成了联苯类化合物抵御外界胁迫;细胞内可溶性蛋白可能作为酶类参与调控化合物的合成;Ca2+信号分子可能介导细胞应对YE胁迫的信号转导。

Suspension cultures cell of Sorbus aucuparia （SASC） was used as materials, the changes of physiological and biochemical indexes of SASC after treatment with yeast extract （YE） were detected, and the synthetic mechanism of secondary metabolites in SASC treated with YE was preliminarily explored. The results were as follows： under the assay conditions, SASC was induced to synthesize five biphenyl compounds, and these compounds content changed differently with induction time prolonging; YE treatment inhibited cell growth, the culture medium pH was gradually reduced after treatment; water-soluble protein content showed a trend of slow decline, which was significantly increased in YE treatment group （YE group） compared with the control group （CK group）, the maximum relative content was 147.76% in contrast with CK group; both YE group and CK group were extracellular Ca^2＋ flow influx, but the YE group flow was significantly slow than CK group. The results indicate that YE induced the cells in a stress state, which was not conducive to the growth of ceils and forced the cells to synthesize biphenyl compounds against external stress ; water-soluble protein may serve as intracellular enzymes involved in the synthesis of compounds regulation; Ca^2＋ may as signal molecule mediate cell signal trans- duction respond to YE stress.