摘要
采用Agilent TC-C18（2）色谱柱（4.6 mm×250 mm，5 μm），以乙腈-水（含0.2%甲酸）为流动相梯度洗脱建立了炙甘草药材的HPLC指纹图谱，并采用HPLC-MS/MS对19个共有峰中的6个色谱峰进行了定性鉴别。采用该方法测定了10批不同产地的炙甘草药材，其相似度在0.72～0.99。该方法精密度、稳定性和重复性良好，该研究为炙甘草药材的质量控制提供了参考。
The chromatographic fingerprint was established by eluting with the mobile phase consisted of acetonitrile and 0.2% formic acid water on an Agilent TC-C18（2） column （4.6 mm×250 mm, 5 μm）. Six chromatographic peaks were identified by HPLC-MS/MS method. Ten batches of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle were determined, and the similarity was arranged from 0.72 to 0.99. Good precision, stability and repeatability were obtained, and this study provides a reference for the quality control of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第11期2056-2059,共4页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2011ZX09201-201-13
2012ZX09301003-001-004
2013ZX09508-105)
关键词
炙甘草
HPLC
指纹图谱
质量控制
Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle
HPLC
fingerprint
quality control
