黄连生物碱对金黄地鼠血脂代谢及低密度脂蛋白受体基因表达的影响

Effects of alkaloids from Coptidis Rhizoma on blood lipid metabolism and low-denstity lipoprotein receptor mRNA in golden hamsters

研究黄连生物碱对低密度脂蛋白受体(LDLR)基因表达和各项降脂指标的影响。通过实时荧光定量PCR检测给药后HepG2细胞和金黄地鼠肝脏中LDLR mRNA的表达量,同时检测给药前后血清总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-c),高密度脂蛋白胆固醇(HDL-c)含量。结果显示,给药后,与模型组相比,各药物组都有降血脂作用,其中黄连碱、巴马汀和药根碱能降低金黄地鼠血清中TC,TG和LDL-c水平(P<0.05,P<0.01),升高HDL-c(P<0.01),增加HepG2细胞和金黄地鼠肝脏中LDLR mRNA的表达量。表明黄连生物碱具有调节金黄地鼠血脂代谢的作用,且黄连碱的降脂效果最好。

To study the effects of alkaloids from Coptidis Rhizoma on low-density lipoprotein receptor（LDLR） mRNA expression and antihyperlipedemic levels. The LDLR mRNA expression were detected by real time fluorescence quantitative PCR, and the levels of total cholesterol（TC）, triglyceride（TG）, low density lipoprotein（LDL-c） and high-density lipoprotein cholesterol（HDL-c） in serum were measured at the first and last examination. The results show that, after the drug treatment, compared with the model group, each drug group showed a lipid-lowering effect. Especially, coptisine, palmatine, jatrorrhinze were significantly reduced TC, TG, LDL-c（P〈0.05, P〈0.01）, and increased HDL-c（P〈0.01）. In addition, they also increased mRNA expression of the LDLR in liver and HepG2 cells. The results showed that alkaloids from Coptidis Rhizoma can regulate lipid metabolism disorder,and coptisine have the best lipid-lowering effect.