乌司他丁对大鼠肝脏冷缺血-再灌注肺损伤的影响

Effect of Ulinastatin on Lung Injury Induced by Liver Cold Ischemia-Reperfusion

目的研究乌司他丁(UTI)预处理对肝缺血-再灌注肺损伤(IRI)的影响。方法将SPF级SD大鼠30只随机分为3组:假手术组(S组,n=10)大鼠仅进行单纯开关腹手术,游离相应血管;模型对照组(M组,n=10)大鼠采用肝脏冷缺血-再灌注模型手术,不给予任何干预措施;UTI组(U组,n=10)大鼠采用肝脏冷缺血-再灌注模型,在切皮时经尾静脉给予UTI,10万U/kg。各组于缺血再灌注后12 h收集血清和肺组织标本,利用ELISA检测TNF-α和IL-6表达水平;切取肺组织,测定肺组织丙二醛(MDA)和超氧化物歧化酶(SOD)含量,光学显微镜下观察肺组织病理学结果。结果与S组相比,M组和U组大鼠血清TNF-α和IL-6浓度及肺组织MDA含量均升高(P<0.05),肺组织SOD活性均降低(P<0.05);与M组相比,U组血清TNF-α和IL-6浓度及肺组织MDA的含量均降低(P<0.05),肺组织SOD活性均升高(P<0.05)。结论 UTI预处理可明显减轻大鼠肝脏冷缺血-再灌注后肺损伤;

Objective To investigate the effect of ulinastatin on lung injury induced by liver ischemia - reperfusion in rats. Methods Thirty healthy male SD rats were randomly divided into 3 groups. In control group （S group）, only abdominal surgery was operated after blood vessels were dissociated. Cold ischemia/reperfusion was performed in Model control group （M group）without any treatment. In the ulinastatin（U group）, the model was the same as M group, while UTI 100 000 U/kg was injected in the tail vein when the skin was cut. After 12 hours, the venous blood was obtained to detect the level of TNF-α and IL- 6. Lungs were removed to detect the level of MDA and SOD in tissue homogenate. The pathological changes of lung were evaluated by light microscopy. Results Compared with M group,the level of MDA, TNF - α and IL - 6 increased statistically in U group （ P 〈 O. 05 ）, the level of SOD decreased statistically in U group （ P 〈 O. 05）, and SOD in lung tissue increased （ P 〈 0.05 ）. Conclusion Ulinastatin can obviously attenuate lung injury after liver ischemia - reperfusion in rats.