汞对大鼠肺泡巨噬细胞TNF-α和NO生成的影响

The effects of mercuric chloride on the production of TNF-α and nitric oxide of the alveolar macrophages originated from different strains of rats

【目的】 比较BrownNorway(BN)和Levis(LE)两种品系大鼠肺泡巨噬细胞产生肿瘤坏死因子 (TNF α)和一氧化氮 (NO)的能力及对氯化汞的反应是否存在差别。【方法】 用肺泡灌洗法收集肺泡巨噬细胞进行培养 ,加入不同浓度的氯化汞 (0 1～ 30 μmol/L) ,处理不同的时间 (12～ 96h)后 ,测定培养基中LDH的相对活性 ,以反映细胞毒性 ,并测定TNF α和亚硝酸盐的含量。【结果】 氯化汞在 3μmol/L以下 ,培养 48h对肺泡巨噬细胞无明显细胞毒性。对照组BN和LW品系TNF α的浓度分别为 (6 0 9 45± 111 94) pg/ml和 (340 6 2± 44 74)pg/ml,两者相比 ,差异有显著性。亚硝酸盐的浓度分别为 (1 86 8± 0 16 5 ) μmol/ml和 (1 313± 0 0 84) μmol/ml,差异也有显著性。氯化汞对TNF α和NO的生成都有一定程度的抑制。【结论】 两种品系大鼠肺泡巨噬细胞体外生成TNF α和NO的量有所不同 ,氯化汞能抑制两种品系大鼠的肺泡巨噬细胞产生TNF α和NO ,抑制作用以BN品系为明显 。

Objective To compare the strain differences of tumor necrosis factor alpha(TNF\|α) and nitric oxide (NO)production of alveolar macrophages(AMs)originated from Brown Norway(BN) and Lewis(LW)rats. Methods The AMs were collected by the bronchoalveolar lavage method,and exposed to concentrations of HgCl\-2(0\^1～ 30 μmol/L ) for fifferent times(12～ 96 h ).The relative LDH activities in the medium were measured to assess the cytotoxicity.The TNF\|α concentration was measured by an ELISA kit and the nitrite was detected with Griess regent. Results The HgCl\-2 was not cytotoxic to the AMs at the concentration of \{3 μmol/L\} within 72 h of incubation.In the control groups the concentrations of TNF\|α and nitrite were (609\^45±111\^94)pg/m and (1\^868±0\^165)μmol/ml,respectively,in BN,which were significantly higher than those in LW strain[(340\^62±44\^7)pg/ml and (1\^313±0\^084)μmol/ml].The production of TNF\|α and NO in both strains were inhibited to some extent by mercuric chloride. Conclusion There are some strain differences in the production of TNF\|α and NO between BN and LW strain of rats.Mercuric chloride inhibited the production of TNF\|α and NO.The inhibitory trends were more serious in BN compared with those in LW.