清营泻瘀方联合力肽对肠缺血再灌注损伤大鼠肠屏障的保护作用

Protective Effects of QXF Combined with Glutamine on Intestinal Ischemia-Reperfusion Injury in Rats

目的:探讨清营泻瘀方联合力肽对肠缺血再灌注损伤大鼠肠屏障的作用及可能机制。方法:SD大鼠72只,随机分为假手术组8只、模型组、中药组(清营泻瘀方)、西药组(力肽)、合用组(清营泻瘀方联合力肽)各16只,后4组按造模后处死时间再分别分为术后2 h组和术后24 h组。采用夹闭肠系膜上动脉45 min的方法诱导肠缺血再灌注损伤,分别观察再灌注后2 h和24 h肠黏膜的病理改变以及血清中TNF-α、IL-10和D-乳酸的水平。结果:清营泻瘀方和力肽都可以减轻肠黏膜的损伤,降低血中IL-10和D-乳酸水平(P<0.05),但清营泻瘀方在再灌注24 h降低IL-10的水平方面优于力肽(P<0.05),清营泻瘀方联合力肽在保护肠黏膜和降低血D-乳酸的水平方面均优于单纯使用清营泻瘀方或力肽(P<0.05)。结论:清营泻瘀方联合力肽对肠缺血再灌注大鼠肠屏障有显著的保护作用,其可能机制与荡涤肠胃宿垢,减少细菌移位,调节炎症介质平衡有关。

Objective To study the effects and mechanisms of QingYingXieYu（清营泻瘀）Fang （QXF） to-gether with glutamine in the protection of gut barrier function of intestinal ischemia-reperfusion in rats.Methods Seventy two SD rats were randomly divided into normal group（n=8）, model group （n=16）, QXF treat-ment group （n=16）, glutamine group （n=16）, QXF combining glutamine treatment group （n=16）.The last fourgroups were also randomly divided into two equal groups after reperfusion at 2 hour and 24 hour respectively.The pathologic injury of the intestines and the levels of TNF-α,IL-10 and D lactic acid in blood were examinedat 2 hours point and 24 hours point after reperfusion respectively.The statistical analysis was finished withSPSS11.0 software. Results The use of QXF and glutamine alone both alleviated the pathologic injury of theintestines, and reduced the levels of IL-10 and D lactic acid. The use of QXF was better than glutamine in re-ducing the level of IL-10. QXF combined with glutamine presented better curative effects than QXF and gluta-mine alone. Conclusion QXF together with glutamine can greatly reduce the gut injury of intestinal ischemiareperfusion with the following possible mechanism：to clear up dunghill in intestine,to regulate the balance of in-flammatory mediators and to protect gut barrier function.