摘要
采用单因素和正交试验方法对超声波辅助提取杜仲籽蛋白工艺进行了优化.先选取料液比、超声时间、超声温度、浸提pH值等条件对杜仲籽蛋白的提取工艺进行单因素实验,采用L9(34)正交试验确定本工艺最佳条件.实验结果表明,在超声波辅助提取杜仲籽蛋白最佳工艺条件(料液比为1∶25,超声时间为30min,超声温度为50℃,浸提pH值为10.0)下,杜仲籽蛋白提取率为77.03%.
The optimum conditions for the ultrasound-assisted extraction of Eucommia seed protein were studied by single factor and orthogonal test method.The solid-liquid ratio,ultrasonic time,ultrasonic temperature,extraction pH value were selected as single factor to study experimental conditions of Eucommia seed protein extraction process,and then optimum process conditions were determined by L9(34)orthogonal test.The experimental results showed that the optimum conditions for ultrasound-assisted extraction of Eucommia seed proteins were as follows:solid to liquid ratio was 1∶25,ultrasonic time 30min,ultrasonic temperature at 50℃,extraction pH value of 10.0.Under this condition,extraction rate of Eucommia seed protein was 77.03%.
出处
《吉首大学学报（自然科学版）》
CAS
2014年第1期78-82,共5页
Journal of Jishou University(Natural Sciences Edition)
基金
湖南省科技厅计划项目(2012J3099)
关键词
杜仲籽蛋白
超声波辅助提取
正交试验
protein of eucommia seed
ultrasound-assisted extraction
orthogonal test
