鳙鱼鱼鳞盐酸脱钙工艺及动力学研究

Studies on process and kinetics of decalcification of bighead carp scale by hydrochloric acid

鱼鳞脱钙是从鱼鳞中提取胶原蛋白的重要的前处理过程。以鳙鱼鱼鳞为对象、盐酸溶液为脱钙剂、羟基磷酸钙消耗速率为目标,采用单因素实验方法分别考察了反应时间、羟基磷酸钙浓度、盐酸溶液浓度和反应温度对羟基磷酸钙消耗速率的影响并确定了最佳工艺条件分别为40 min、0.017 4 mol·L-1、0.5 mol·L-1和20℃。在最佳工艺条件下,羟基磷酸钙消耗速率为3.744 9×10-4mol·L-1·min-1,相应的脱钙率为86.20%。建立了盐酸脱钙的动力学方程,模型反映了羟基磷酸钙消耗速率随羟基磷酸钙浓度、盐酸溶液浓度与温度的变化关系,其理论值与实验值基本吻合,可用于过程调节、产品控制和预测不同条件下羟基磷酸钙的反应消耗速率。

Decalcification of fish scale is an important pre-treatment process for collagen extraction from fish scale. With bighead carp scale as research objective, hydrochloric acid solution as decalcification agent and hydroxyapatite consumption rate as target, the single experiment method was used to investigate the effects of reaction time, hydroxyapatite concentration, hydrochloric acid concentration and reaction temperature on hydroxyapatite reaction consumption rate and their optimal values were determined to be 40 min,0.017 4mol·L-1,0.5mol·L-1 and 20℃ ,under which the hydroxyapatite reaction consumption rate reached 3. 744 9×10^-4mol·L-1·min-1 with corresponding decalcification rate of 86.20%. The kinetic equation of the decalcification was established, which reflected the change relations of hydroxyapatite reaction consumption rate with hydroxyapatite concentration, hydrochloric acid concentration and temperature. The model values were basically agreed with experimental ones, which can be used for decalcification process adjustment, product control and prediction of hydroxyapatite consumption rate at different conditions.