外阴阴道念珠菌病复发与阴道及肛管白念珠菌定植关系的研究

Analysis on the correlation between recurrent vulvovaginal candidiasis and Candida Albicans planted in vagina and anal canal.

目的从致病菌角度分析复发性外阴阴道念珠菌病(RVVC)复发的危险因素。方法收集30例RVVC患者阴道和肛管的菌株,经白念珠菌特异性引物PCR鉴定有17例为白念珠菌并入选研究。三色荧光分别标记保守基因CDC3、EF3和HIS3微卫星序列引物,PCR扩增,产物行聚丙烯酰胺凝胶电泳。Gene Scan软件扫描获取片段精确长度,Genotype软件基因分型。结果分离自17例患者阴道和肛管的白念珠菌,采用微卫星基因分型法,基因型彼此相同。结论 RVVC患者阴道的菌株可能从肛管移行而来,这可能是该病反复发作的重要因素之一。

Objective To analyze the risk factors of pathogens in relapse of recurrent vulvovaginai eandidiasis （RVVC）. Methods Candida strains were isolated from the vaginas and anal canals of 30 patients with RVVC. Candida strains from 17 patients were identified as eandida albicans by specific primers. The house keeping genes CDC3, EF3 and HIS3 was featured with microsatellite sequence. The 3 sites of house keeping gene were amplified by specific primers, which were labeled with different fluorescein. PCR products were run on polyacrylamide gel and sizes of products were scanned by GeneScan software and identified by Genotype software. Results The genotypes of 17 pairs of candida albicans strains were same. Conclusion In patients with RVVC, the strains could migrate form anal canal to vagina, which might be the important causes for the recurrence of RVVC.