碱性蛋白酶水解欧李仁多肽的研究

Hydrolysis of Cerasus humilis Kernel Polypeptide with Alcalase

试验以欧李仁粕为原料,从中提取蛋白质,在此基础上,选取碱性蛋白酶食品级蛋白酶,对欧李仁蛋白质进行单酶分步水解,通过测定水解度的方法,研究碱性蛋白酶对欧李仁多肽的水解效果,并探讨水解时间、底物浓度、pH、酶添加量对该碱性蛋白酶水解效果的影响。通过正交试验和极差分析确定了碱性蛋白酶水解欧李仁多肽的最佳工艺条件为:底物浓度3%,pH 10,酶的添加量为1.5%,水解时间为20 min。

Take Cerasus humilis Kernel as a raw material and extract protein from it. The single-enzyme hydrolysis assay wasapplied for Cerasus humilis Kerne peptide preparation. The Alcalase was used in the study. Effect of Alcalase on thehydrolysis of Cerasus humilis Keme polypeptide was studied by determination of its degree of hydrolysis （DH）. Parametersinfluencing the Alcalase hydrolysis, including hydrolysis time, ratio of enzyme and substrate concentration, pH, substrateconcentration were also investigated. The optimum combination of parameters conditions through orthogonal experiment andthe range analysis were determined. The results were as follows the reaction time was 20 min, substrate concentrationwas 3%, pH was 10, and ratio of enzyme and substrate concentration was 1.5%.