哮喘患儿血管紧张素转移酶基因I/D多态性分析

Detection of insertion/deletion(I/D) polymorphism of angiotensin-coverting enzyme gene in children with asthma.

目的 :探讨血管紧张素转移酶 (ACE)基因插入 /缺失 (I/D)多态性与儿童哮喘的关系。方法 :采用聚合酶链反应(PCR)方法检测 5 2例哮喘患儿 ,40例正常儿童的ACE基因型。结果 :哮喘组DD基因型频率和D等位基因频率分别为 35 %和 45 % ,而正常对照组为 13%和 31%。两组比较有显著性差异 (P <0 .0 5 ) ,携带DD基因型与非DD基因型的哮喘患儿间最大呼气流量占预计值的百分比无显著性差异 (P >0 .0 5 )。结论 :ACE基因DD基因型与哮喘的易感性有关 ,可能是哮喘的危险因素 ,而与气道阻塞程度无关。

To assess the relationship of the I/D polymorphism of ACE gene to asthma in children.Methods: ACE genotypes were performed by polymerse-chanin-reaction (PCR) technique in 52 asthmatic children and 40 healthy subjects.Results:There was an high frequency of DD genotype and D allele of ACE gene in asthmatic children compared with healthy subjects (35% vs 13%,45% vs 31%,P<0.05),and asthmatic children were classified into two groups (DD or non-DD) according to ACE genotype to compare their PEFR ratio,no differences were found (P>0.05).Conclusion:DD genotype ACE gene are related to the susceptibility of asthma and may be a risk factor in the development of asthma.DD genotype of ACE gene is not associated with the degree of airway obstruction in children with asthma.