核心蛋白聚糖在Ishikawa细胞中的表达及意义

Expression and significance of decorin in Ishikawa cells

目的观察表皮生长因子受体(EGFR)信号通路抑制剂RG-14260(RG)单用及RG联合mTOR信号通路抑制剂亚罗莫司(RA)体外对子宫内膜癌Ishikawa细胞增殖及核心蛋白聚糖(DCN)表达的影响。方法子宫内膜癌Ishikawa细胞经RG及RG联合RA作用后,采用免疫组织化学法检测细胞增殖细胞核抗原(PCNA)的表达,反转录酶-聚合酶链式反应(RT-PCR)及免疫印迹法分别检测细胞内DCN基因和蛋白表达的变化。结果 RG单用及联合RA均可降低Ishikawa细胞PCNA阳性细胞表达率,促进细胞DCN基因和蛋白的表达,但二者合用效果更为显著。结论单独和联合EGFR及mTOR信号通路抑制剂均可在基因和蛋白水平上上调Ishikawa细胞内DCN的表达,而升高的DCN反过来又可增强信号通路抑制剂对细胞增殖的抑制作用。

Objective To observe the effect of epidermal growth factor receptor （EGFR） signal transduction inhibitor RG-14260 （RG） alone or combined with roTOR signal transduction inhibitor rapamycin （RA） on the proliferation and expression of decorin （DCN） in Ishikawa cells in vitro. Methods After treated with RG alone or combined with RA for 24 hours, immunohistochemical method was used to detect the expression of proliferating cell nuclear antigens （PCNA）. Reverse transcriptase-polymerase chain reaction （RT-PCR） and Western blot method were used to detect the expression changes of DCN genes and proteins in ceils respectively. Results RG alone or combined with RA were able to decrease the expression rate of positive PCNA ceils in Ishikawa cells, promote the expression of DCN genes and proteins, but the efficacy of combined application was more significant. Conclusion Single and combined applications of EGFR and roTOR signal transduction inhibitors can upthe regulate the expression of DCN in Ishikawa cells at the levels of genes and proteins, and meanwhile increased DCN can reinforce the inhibitory effect of signal transduction inhibitors on the cell proliferation.