肝脏去交感神经状态对大鼠脂类和激素代谢的影响

Influence of denervation of sympathetic nerve on metabolism of lipid and hormone in rats

目的探讨在大鼠肝损伤状态下化学性去除肝脏交感神经对肝脏脂类和激素代谢功能的影响。方法应用CCl_4制作大鼠急性肝损伤模型,6-OHDA制作大鼠去交感神经模型,实验组进行"去交感神经"预处理后应用CCl_4致"急性肝损伤",对照组进行假手术处理。处理7 d后,检测不同处理组的动物血清中脂类代谢和激素代谢指标[甘油三酯(TG)、总胆固醇(Tch),血清皮质醇(Cor)、血清醛固酮(ALD)、免疫活性胰岛素(IRI)]。结果急性肝损伤后,肝脏功能明显下降,肝损伤模型组TG(0.87±0.09)μmol/L、Tch(1.47±0.21)μmol/L与对照组TG(1.22±0.18)μmol/L、Tch(2.37±0.29)μmol/L相比明显下降(P<0.05)。实验组TG(1.05±0.04)μmol/L、Tch(1.96±0.06)μmol/L与肝损伤模型组比较有所升高。肝损伤模型组Cor(28.3±5.6)μg/L、ALD(651.6±82.3)ng/L较对照组Cor(9.5±3.4)μg/L、ALD(196.5±47.3)ng/L明显升高(P<0.05),实验组Cor(20.9±4.2)μg/L、ALD(471.73±72.9)ng/L较肝损伤模型组均明显降低(P<0.05)。肝损伤模型组的IRI(20.37±5.57)mIU/L较对照组(12.48±3.03)mIU/L明显升高(P<0.05),而实验组的IRI(29.39±7.67)mIU/L较对照组和肝损伤模型组均明显升高(P<0.05)。在大鼠肝损伤状态下,去除交感神经能够促进肝脏脂类合成和激素的代谢,也使胰岛素分泌增多。结论去交感神经能够改善急性肝损伤时的肝脏功能。

Objective To explore the influence of sympathetic denervation of the liver on me-tabolism of lipid and hormone in the normal and chemically-injured liver in rats.Methods Liver in-jured rats were obtained by using CCl4 .Denervation of sympathetic nerve was achieved by injection of 6-OHDA （2 mL,100 mg/L）and the mesenteric vein of jejunum loop.Experimental group was treated with CCl4 after injecting 6-OHDA.Control group was treated with a sham operation.After 7 days,the serum levels of triglyceride （TG）,total cholesterol （Tch）,cortisol（Cor）,aldosterone（ALD）and im-munoreactive insulin（IRI）were detected in every group.Results The liver function of the rats with acute liver injury decreased.After the denervation of sympathetic nerve,the serum levels of TG and Tchin the experimental group were higherthan the liverinjury model group [Control group ：TG（1 .2 2 ± 0.18）μmol /L,Tch（2.37 ±0.29）μmol /L,liver injury model group：TG （0.87 ±0.09）μmol /L, Tch（1 .47 ±0 .2 1 ）μmol /L（P 〈0 .05 ）.Experimentalgroup：TG（1 .05 ±0 .04 ）μmol /L,Tch （1.96 ±0.06）μmol /L].Compared with the control group,the serum levels of Cor and ALD in the experimental group and the liver injury model group increased significantly[Control group：Cor（9.5 ± 3.4）μg/L,ALD（196.5 ±47.3）ng/L.Liver injury model group：Cor（28.3 ±5.6）μg/L,ALD （65 1 .6 ±82 .3 ）ng /L.Experimentalgroup ：Cor（20 .9 ±4 .2 ）μg /L,ALD（47 1 .73 ±72 .9 ） （P 〈0 .05 ）].SerumlevelofIRI inthe liverinjurymodelgroupwas（20 .37 ±5 .5 7 ）mIU /L,which was significantly higher than （12.48 ±3.03）mIU /L in the control group（P 〈0.05）.Simultane-ously,the serum level of IRI in the experimental group was（29.39 ±7.67）mIU /L,which was sig-nificantly higher （P 〈0.05）than both the blank control group and the liver injury model group. Sympathetic denervation had a beneficial effect on the anabolism of TG and Tch,metabolism of ALD and Cor.Conclusion Sympathetic denervation can promote the recovery of liver function in acute chemically-injured liver.