VEGF及DLL4在肝细胞癌中的表达及临床意义

Expression and clinical significance of VEGF and DLL4 in hepatocellular carcinoma

目的通过对肝细胞肝癌组织中VEGF和DLL4表达水平的测定,分析其与临床病理分期的关系及意义。方法2010年1月—2013年6月本院外科手术切除并经病理证实的肝细胞肝癌组织石蜡标本75例,采用免疫组化SP法测定75例肝细胞肝癌及30例癌旁肝组织中VEGF和DLL4的表达水平结果 VEGF和DLL4在肝细胞肝癌组织中表达的阳性率分别为64.0%、81.3%,明显高于癌旁组织(P<0.05)。VEGF及DLL4在肝细胞肝癌中的表达与肿瘤数目、有无静脉浸润密切相关(P<0.05);两者在肝细胞肝癌组织中的表达与患者性别、年龄、肝硬化程度、肿瘤直径和分化程度无关(P>0.05)。VEGF阳性表达患者中的DLL4表达率明显高于VEGF阴性表达患者,VEGF和DLL4表达水平呈显著正相关(r=0.217,P<0.05)。结论 VEGF和DLL4在肝细胞肝癌和正常肝细胞中的表达差异有统计学意义,两者在肝细胞肝癌中表达上调具有一致性,共同促使肝癌新生血管生发,参与肝细胞肝癌的生长和转移,影响患者的预后。

Objective To explore the expression of VEGF and DLL4 in hepato cellular carci-noma tissues.Methods Expressions of VEGF and DLL4 in 75 cases of hepatocellular carcinoma tis-sues and 30 cases of normal hepatocellular mucosa tissues were detected by immunohistochemical SP method.Results The masculine expression rates of VEGF and DLL4 in hepatocellular carcinoma tis-sues were 64.0% and 81.3% respectively.The positive expression rates of VEGF and DLL4 in hepa-tocellular carcinoma were higher than those in normal hepatocellular mucosa.Expressions of VEGF and DLL4 in hepatocellular carcinoma tissues were associated with vascular invasion and tumor numbers（P〈0.05）,but not with gender,age,liver cirrhosis,the diameter of tumor and the histological differen-tiation（P 〉0.05 ）.The VEGF expression rate in DLL4 positive expression cases was significantly higher than DLL4 negative cases.VEGF had a positive correlation with DLL4 （r =0.217,P 〈0.05）. Conclusion The expressions of VEGF and DLL4 in hepatocellular carcinoma and normal tissues are different.The increased expression of VEFG in hepatocellular carcinoma is closely related to DLL4 and they probably play an important role in tumor angiogenesis.