T细胞亚群在原发性干燥综合征患者外周血中的表达水平及其意义

Expression level and significance of T cell subgroups in the peripheral blood of patients with primary Sjogren's syndrome

目的探讨T细胞亚群在PSS患者外周血中的表达水平及其意义。方法采用流式细胞仪对32名原发性干燥综合征(PSS)患者及35名健康体检者外周血的CD 3 +、CD 3 +CD 4 +CD 8 -、CD 3 +CD 4 -CD 8 +、CD 3 +CD 4 -CD 8 -及CD 4 +CD (25) (+high)表达水平进行检测,并进行组间比较。结果 2组患者的CD 3 +、CD 3 +CD 4 -CD 8 +水平无显著差异(P>0.05),观察组的CD 4 +/CD 8 +、CD 3 +CD 4 +CD 8 -亚群的比例较观察组有明显升高(P<0.01),CD 3 +CD 4 -CD 8 -双阴性T细胞与CD 4 +CD (25) (+high)亚群的比例较观察组显著下降(P<0.01)。结论 PSS患者的T细胞亚群表达水平异常,过多的CD 4 +细胞增殖与浸润会成为发生自身免疫性疾病的危险因素,CD 3 +CD 4 -CD 8 -双阴性T细胞与CD 4 +CD (25) (+high)亚群水平的下降会造成对机体免疫耐受状态的破坏,可能是引发PSS发生的重要因素之一。

Objective To explore the expression levels and significance of T cell subgroups in the peripheral blood of patients with primary Sjφgren′s syndrome （PSS）.Methods Flow cytometer was applied to detect CD3^＋,CD3^＋CD4^＋CD8^-,CD3^＋CD4^-CD8 ＋,CD3^＋CD4^-CD8^- and CD4^＋CD25^＋high expression levels in 32 PSS patients （observation group）and another 35 healthy people tak-ing physical examination （control group）,which were then compared between groups.Results There were no significant differences between two groups in CD3^＋ and CD3^＋ CD4^- CD8 ＋ expressions （P 〉0.05）.The ratios of CD4^＋/CD8 ＋ and CD3^＋CD4^＋CD8^- subgroups increased evidently （P 〈0.01）, while those of CD3^＋ CD4^- CD8^- double-negative T cells and CD4^＋ CD25^＋high subgroup decreased markedly in observation group （P 〈0.01）.Conclusion T cell subgroup expressions are abnormal in PSS patients,and over proliferation and infiltration of CD4^＋ cells is the risk factor of auto-immunologi-cal diseases,while the decrease of CD3^＋CD4^-CD8^- double-negative T cells and CD4^＋CD25^＋high sub-group can lead to the damage of body immunological tolerance,which may be one of the important fac-tors for the development of PSS.