依达拉奉对犬自体肾移植外周血中炎性因子的影响

Influence of edaravone on inflammatory factor in peripheral blood in canine with renal auto-transplantation

目的探讨依达拉奉对犬自体肾移植外周血中炎性因子的影响。方法将12只体质量匹配的健康杂种犬随机分为依达拉奉组(ED组)和生理盐水组(PS组),每组6只。ED组术中静脉内给予依达拉奉10 mg/kg,术后第1、2、3天各予依达拉奉10 mg/kg;PS组同法使用相同体积的生理盐水。分别在麻醉前(T_0)、术中(T_1)、术后1 d(T_2)与术后3 d(T_3)经静脉采血检测TNF-α、IL-6、IL-8、IL-10的水平。结果 2组外周血T_0期比较各因子浓度无显著差别;T_1、T_2、T_3各期TNF-α、IL-6、IL-8、IL-10浓度较T_0期显著升高(P<0.05),T_1期剧升,T_2期达到高峰,T_3期略回落但仍高于T_0期;ED组TNF-α、IL-6、IL-8升高的幅度在T_1、T_2、T_3期小于PS组(P<0.05);2组IL-10比较无显著差异(P>0.05)。结论肾移植炎性因子显著升高,可能与肾移植缺血再灌注损伤有关;依达拉奉能减少炎性因子的释放,亦是其肾保护作用的机制。

Obj ective To explore the influence of edaravone on inflammatory factor of pe-ripheral blood in canine with renal auto-transplantation.Methods 12 weight matched healthy mon-grel dogs were randomly divided into edaravone group （ED group ）and normal saline group （PS group）,6 dogs in each group.ED group was treated with edaravone by 10 mg/kg intravenous in in-traoperative,postoperative 1,2,3 days,and each for edaravone 10 mg/kg.PS group was treated with the method of using the same volume of saline.The levels of TNF-α,IL-6,IL-8,IL-10 were detected by sampling venous blood before anesthesia （T0 ）and after surgery （T1 ）,1 days （T2 ）and 3 days （T3 ）after operation respectively.Results Compared with levels of inflammatory factors of peripheral blood in T0 period,there were no significant differences of concentration in each factor. With increasing of concentration of IL-6,IL-8,TNF-α,IL-10 at T1,T2,T3（P〈0.05）,the lev-els raised at T1 ,reached a peak at T2 ,slightly down at T3 but still higher than T0 .Levels of TNF-α,IL-6 ,IL-8 in ED group increased in T1 ,T2 ,T3 were significantly less than the PS group （P〈0.05）.There was no significant difference of IL-10 between two groups （P〉0.05）.Conclusion Inflammatory factors significantly increase in dogs with renal allograft,and it may be associated with ischemia reperfusion inj ury in renal transplantation.Edaravone can reduce the release of in-flammatory factors,which is also its mechanism of renal protection effect.