结扎孕鼠双侧子宫动脉复制围产期缺氧缺血性脑损伤动物模型

Reconstructing new animal model of peri-delivery hypoxia ischemia brain damage by ligaturing bilateral uterine arteries of pregnant mouse

目的 制作一种新型围产期缺氧缺血性脑损伤（HIBD）的动物模型，为进一步深入研究其病理生理机制及治疗方法提供条件。方法 结扎足月妊娠待产（妊娠19.5d）昆明母鼠双侧子宫动脉，不同时间行剖宫产娩出胎鼠,与正常剖宫产娩出的胎鼠相比较，观察实验胎鼠的生长发育及脑部病理改变。结果 随着结扎子宫动脉阻断血供时间的延长，胎鼠的死亡率迅速增高，两者具有直线正相关关系（P＜0.05），实验组胎鼠体重增长明显减慢，运动发育迟滞，脑部的病理改变与人类HIBD的改变相一致。结论 通过结扎孕鼠双侧子宫动脉，胎鼠所产生的一系列变化符合人类HIBD的改变，这是一种较理想的、操作较简便的复制HIBD动物模型的方法。

Objective Hypoxia-ischemia brain injury (HIBD ) in infants is one of the important factors which induce intelligent defect,cerebral palasy in human beings. Therefo re, we produced a new animal model to study HIBD. Method We assigned twenty 19.5 pregnancy day mice to four groups randomly and had ligated their bilateral uteral arteries for 0,10,20, and 30 minutes respectively before the cesarean sections. Of each group,we obse rved the mortality,the growth and the brain's pathological changes. R esult The longer the uteral arteries were ligated,the higher the mortality was. The slowlier the body weigh t increased, the more serious the spastic paralysis was .We also found the mice is similar in the brain pathological change to the human beings such as the edema regions,spotted hemorrhage,and local malacosis in the cortex. Conclusion This is a simpl e and useful model to study HIBD in newborn.