BMP、IL- 1、IL- 6、TNF-α在先天性胫骨假关节中的表达

Immunohistochemical study of the expression of BMP,IL－ 1,IL－ 6,TNF－α in congenital pseudarthrosis of tibia

目的了解先天性胫骨假关节 (congenital pseudarthrosis of tibia， CPT)中骨形态发生蛋白 (bone morphogenetic proteins,BMP)、白细胞介素 1(interleukin－ 1,IL－ 1)、白细胞介素 6(interleukin－ 6,IL－ 6)、肿瘤坏死因子 (tumor necrosis factor,TNF－α )的表达，探讨它们在 CPT发病机制中的作用。方法采用免疫组化方法对 24例 CPT患者的 25例病变骨膜标本中 BMP、 IL－ 1、 IL－ 6、 TNF－α的表达水平进行检测。与正常骨膜 (10例 )，创伤性假关节 (traumatic pseudarthrosis， TP)骨膜 (20例 )和纤维瘤病组织 (10例 )对照。结果所有正常骨膜标本中 BMP染色均为阳性， TP骨膜中 8例标本 BMP染色阳性，而 CPT骨膜和纤维瘤病病变组织中未见 BMP染色阳性 ,与正常骨膜和 TP骨膜相比差异有显著性意义 (P< 0.05)；正常骨膜、 TP骨膜中未见 IL－ 1、 IL－ 6、 TNF－α染色阳性，而 CPT骨膜和纤维瘤病病变组织中均有部分标本染色阳性，与正常骨膜及 TP骨膜相比差异有显著性意义 (P< 0.05)。结论 BMP、 IL－ 1、 IL－ 6、 TNF－α的异常表达在先天性胫骨假关节发病中起重要作用。

Objective To investigate the expression of BMP, IL－ 1, IL－ 6, TNF－α and detect their function in congenital pseudarthrosis of tibia (CPT). Methods Twenty- five specimens were procured from 24 CPT patients. Streptoavidin Peroxidase are applied to determine the expression of level of BMP, IL－ 1, IL－ 6,TNF－α . The control specimens were taken from normal periosteum in 10 cases, 10 cases of fibromatosis and 20 cases of traumatic pseudarthrosis. Results All specimens of normal periosteum were positive for BMP. 8 specimens of traumatic pseudarthrosis were positive for BMP, but all specimens of traumatic pseudarthrosis were negative for IL－ 1,IL－ 6, TNF－α . Some specimens of CPT and fibromatosis were positive for IL－ 1,IL－ 6,TNF－α ,but all specimens of CPT and fibromatosis were negative for BMP. There was no significant difference between the periosteum of CPT and fibromatosis in expression level of IL－ 1, IL－ 6, TNF－α (P >0.05). The expression of IL－ 1, IL－ 6, TNF－α in fibromatosis and the periosteum of CPT was higher than that in normal periosteum and traumatic pseudarthrosis (P< 0.05). Conclusion High expression of IL－ 1, IL－ 6, TNF－α plays an important role in the pathogenesis of CPT.