过氧化氢对体外培养的兔晶体上皮细胞凋亡的诱导

Apoptosis of rabbit lens epithelial cells induced by H_2O_2

目的 :研究 H2 O2 诱导兔晶体上皮细胞凋亡。方法 :采用终浓度 2 5 0 μmol/ L H2 O2 处理兔晶体上皮细胞 ,分别用 TUNEL法、流式细胞仪和透射电镜检测 H2 O2 处理后的兔晶体上皮细胞。结果 :用 H2 O2 处理后的兔晶体上皮细胞 ,TUNEL 法可见胞核呈棕褐色着染 ,苏木素不能复染 ;流式细胞仪检测到约 9.6× 10 5 细胞出现亚二倍体峰 ,二倍体峰几近消失 ;透射电镜下细胞膜完整 ,但表面微绒毛消失 ,细胞质浓缩 ,胞浆内细胞器结构不清晰 ,并见大量大小不等的空泡 ,核质比例增大 ,胞核呈圆形 ,核膜双层结构不清 ,呈光滑状 ,核内染色质聚集成块 ,部分染色质边集。结论 :终浓度 2 5 0μmol/ L H2 O2

Objective:to investigate the inducing significance of H 2O 2 on the apoptosis of rabbit lens epithelial cells(LEC).Methods:rabbit lens were incubated in vitro for 24 hours with 250μmol/L H 2O 2,then the epithelial cells were collected and examined with TUNEL,Flow Cytometry and transelectroscope.Results:The cellular nuclei of rabbit LEC were stained in dark brown after treeted with H 2O 2 and could not be restrained with hematoxylin under TUNEL.Sub diploid peak which contained 9 6×10 cells was detected by flow cytometry and diploid peak almost disappeared.Under transelectroscope it could be observed that cellular membrane was intact,cytoplasm was shrinkage,nucleus proportion increased,structures of cellular organs were unclear,and chromatin gathered in the peripheral nucleus.Conclusion;250μmol/L H 2O 2 can induce the apptosis of rabbit LEC.