实验性脑缺血再灌流后Bak和Bad基因在脑内的表达

Expression of Bak and Bad gene in brain after global ischemia-reperfusion

目的：观察全脑缺血再灌流后Bak和Bad基因在脑内表达的变化规律和意义。方法：采用大鼠4血管结扎全脑缺血30min再灌流模型，用免疫组织化学染色法观察Bak和Bad基因在脑内的表达。结果：全脑缺血再灌流后12h开始在皮质和海马等缺血易损部位：Bak表达降低，24h达最低点；而Bad则表达升高，24h达最高点；表达变化最明显的时间均是24～48h，此时间与脑缺血后脑细胞凋亡发生的高峰时间一致。结论：脑缺血再灌流可以诱导Bak和Bad基因在脑内表达发生变化，这种变化与脑细胞凋亡的发生关系密切。Bak在脑缺血后表达降低说明其在脑内表达可抑制细胞凋亡。

Objective: To investigate the expression of Bak and Bad gene after ischemia-reperfusion. Methods: Ischemia was induced by the four-vessel occlusion for 30 min and followed by reperfusion in rats. The biopsy tissue from brain was immunohistochemically assayed with Bak and Bad genes polyclonal antibody . Results: The expression of Bak protein decreased as early as 12h after onset of reperfusion and peaked at 24~48h. At the same time, however, the expression of Bad protein increased and peaked at 24~48h. The expression of Bak and Bad gene was quite clear in cortex and hippocampus CA1 which were the sensitive areas for ischemic injury. Conclusion: The ischemia-reperfusion induced the changes of expression of Bak and Bad gene in brain which may accelerate apoptosis of neurons.