外周血白蛋白mRNA分析在肝癌诊断和转移监测中的价值

Analysis of peripheral blood albumin gene fragment and its clinical value in diagnosis and monitoring metastasis of hepatocellular carcinoma

目的 探讨外周血有核细胞中白蛋白 (Alb)基因在肝癌诊断和转移监测中的价值。方法 以逆转录巢式PCR法扩增肝癌组织及外周血中Alb基因片段 ,分析其在肝癌诊断和转移监测中的价值。结果 2 0份肝癌、癌周和远癌组织总RNA浓度 ,表现为远癌和癌周组织明显高于癌灶组织 (P <0 0 5 )。Alb基因检出率在所有肝组织均为 10 0 % ,在肝癌患者外周血中为 5 9 7%。明显高于肝硬化、慢性肝炎和肝外肿瘤组患者 (P <0 0 1) ,但与急性肝炎组相比未见差别 (P >0 0 5 ) ;Alb基因在Ⅰ、Ⅱ和Ⅲ期肝癌中阳性率分别为 33%、37%和77% ;在肝内有无转移组间差异明显 (P <0 0 5 ) ,在伴肝外转移肝癌中全数阳性 ;在AFP <5 0 μg/L肝癌中阳性率为 17% ;Alb基因检出率与肝癌大小间无明显差异 (P >0 0 5 )。结论 外周血肝细胞Alb基因分析为肝癌诊断、转移和监测术后复发的有用标志物。

Objective To explore the peripheral blood albumin gene marker in clinical value of diagnosis and monitoring metastasis of hepatocellular carcinoma(HCC).Methods The albumin gene was amplified by nested PCR and its clinical value was investigated in liver tissues and peripheral bloods(PB) from patients with HCC.Results Of the 20 hepatoma tissues, total RNA concentrations in paracancerous and distal cancerous tissues were significantly higher than that in cancerous tissues(P<0 05). The incidence of AlbmRNA was 100% in different liver tissues, 59 7% in PB from patients with HCC. The latter was significantly higher than that in LC, CH and extrahepatic tumor(P<0 01), but no remarkably difference was found between HCC and AH groups. The incidence of Alb-mRNA was 33% in I stage HCC, 37% in Ⅱstage HCC and 77% in Ⅲstage HCC. Significant difference(P<0 05) was found between 100% in cases with extrahepatic metastasis and 17% in cases with AFP less than 50μg/L. No correlation was found between Alb gene and tumor size.Conclusion The analysis of peripheral blood Alb gene is a useful marker for HCC diagnosis and monitoring metastasis and recurrence of HCC.