HPLC测定跌打丸中人参皂苷Rg_1的含量

Content Determination of Ginsenoside Rg_1 in Dieda Pills by HPLC

目的：建立跌打丸中人参皂苷Rg1的含量测定。方法：色谱柱为Agilent ZORBAX C18（4.6 mm ×250 mm，5μm），流动相为乙腈-水（78∶22）；流速：0.8 mL/min，检测波长为203 nm，柱温为30℃。结果：人参皂苷Rg1浓度在255.40～766.20μg/mL与峰面积有良好的线性关系（r =0.9985），平均回收率为92.8%（n =6）， RSD为0.58%。结论：该方法快速、准确、重现性好，可作为跌打丸的质量控制方法。

Objective：To establish a HPLC method to determine ginsenoside Rg1 in dieda pills. Methods：The column of Agilent ZORBAX C18（4.6 mm × 250 mm,5 μm）was used with acetonitrile-water （78：22） as mobile phase, the flow rate was 0.8 mL/min, the detection wavelength was at 203 nm and the column temperature was at 30 ℃. Results：There was a good linear relationship between ginsenoside Rg1 at the concentration of 255.40 ~ 766.20 μg/mL and the peak area （ r = 0 . 998 5 ） , and the average recovery was 92 . 8% （ n = 6 ） , RSD was 0 . 58%. Conclusion：The method is simple , sensitive and accurate . It can be used for the quality control of dieda pills .