HPLC法同时测定一枝黄花药材中绿原酸、芦丁和山奈素的含量

HPLC Determination of Chlorogenic Acid, Rutin and Kaempferide in Solidago decurrens Lour

目的：建立同时测定一枝黄花药材中绿原酸、芦丁和山奈素含量的高效液相色谱法。方法：采用Diamonsil C18柱（4.6 mm＆#215;200 mm，5μm），流动相乙腈-0.2%磷酸水溶液梯度洗脱，流速1.0 mL·min-1，柱温25℃，检测波长282 nm，柱温25℃。结果：绿原酸、芦丁和山奈素的回归方程分别为Y=1712.1X＋11.686（r=0.9993）、Y=13584.0X＋13.984（r=0.9994）、Y=5971.2X＋4.6175（r=0.9998）；线性范围分别为63.2-442.4μg、8.1-56.8μg、10.8-75.7μg；回收率分别为98.6%（RSD=1.4%）、99.2%（RSD=0.8%）、100.3%（RSD=1.0%）。结论：本法简便快捷，重复性好，可用于一枝黄花中绿原酸、芦丁和山奈素的定量分析。

This study was aimed to establish an HPLC method for the determination of chlorogenic acid, rutin and kaempferide in Solidago decurrens Lour. A Diamonsil C18 column （4.6 mm ＆#237; 200 mm, 5 μm） was adopted with the mobile phase of acetonitrile-0.2% phosphoric acid. The flow rate was 1.0 mL·min-1. The column temperature was at 25℃. The wavelength of detection was set at 282 nm. The results showed that the calibration curve was linear over the range of 63.2-442.4 μg for chlorogenic acid （r = 0.999 3）, 8.1-56.8 μg for rutin （r = 0.999 4） and 10.8-75.7μg for kaempferide （r = 0.999 8）. The average recovery of chlorogenic acid was 98.6% （RSD = 1.4%）, that of rutin was 99.2% （RSD = 0.8%） and that of kaempferide was 100.3% （RSD = 1.0%）. It was concluded that the method was simple, economical and accurate with good reproducibility.