基于碳纳米角-阿霉素的新型载药体系的制备和体外释放行为

Preparation and in vitro release behavior of a new drug delivery system based on single-walled carbon nanohorns-doxorubicin

目的制备一种以壳聚糖(CS)为修饰剂氧化单壁碳纳米角(oxSWCNHs)介导的载阿霉素(DOX)的新型药物转运系统(DOX@oxSWCNHs/CS),并考察理化性质及体外释放行为。方法制备DOX@oxSWCNHs/CS载药体系,考察体系在水、PBS、细胞培养基中的分散性,使用热重分析(TGA)、透射电镜(TEM)、紫外可见吸收光谱、荧光光谱、zeta电位对其理化性质进行考察,评价其体外释放效果。结果 DOX通过π-π堆积作用装载于oxSWCNHs上,载药量达60%;用CS修饰oxSWCNHs的疏水表面,可增加oxSWCNHs在水溶液中的分散性,特别是在盐溶液中分散性。载药体系的体外释放具有pH依赖性和缓释效果。结论 oxSWCNHs能够作为一种潜在的阿霉素载体达到药物的缓释效果。

Objective To prepare and characterize a new oxidized single-wall carbon nanohorns （oxSWCNHs） -mediated drug delivery system （DOX@oxSWCNHs/CS） which loads doxorubicin （DOX） and is modified by chitosan （CS）, and evaluate their in vitro release quality. Methods DOX@oxSWCNHs/CS nanocarrier was prepared and the dispersity in water, PBS, and cell culture medium was estimated. The characteristics of DOX@oxSWCNHs/CS were evaluated by the thermogravimetry analysis （TGA）, transmission electron microscopy （TEM）, UV-vis absorbance spectra, fluorescence spectra and zeta potential. Drug release behaviors were also investigated. Results DOX was attached to oxSWCNHs surface via π-π stacking interaction, the weight percent of DOX in DOX@oxSWCNHs was 60%, followed by encapsulation of oxSWCNHs with CS to enhance the stability of nanocarrier in the aqueous medium, especially in the salt solution. The release of drug on nanocarrier was pH-dependent and controlled. Conclusion oxSWCNHs can act as a potential DOX carrier for sustained drug release.