可溶性人类白细胞抗原-G1 cDNA的克隆及序列分析

Molecular cloning and sequencing of the cDNA of the soluble human leukocyte antigen-G1

目的建立表达可溶性 HL A- G1蛋白的真核表达载体 pc DNA3- s HL A- G1。方法从绒癌细胞系 Jeg- 3细胞中提取总 RNA,借助 RT- PCR技术扩增可溶性 HL A - G1的 c DNA并把其插入真核表达载体 pc DNA 3,然后经酶切和测序法鉴定。结果经酶切鉴定及测序分析 ,证实已成功构建 pc DNA3- s HL A- G1。结论本研究成功构建可溶性 HL A -

ObjectiveTo construct a recombinant plasmid pcDNA3 sHLA G1 expressing soluble HLA G1. Methods Total cell RNA was extracted from the cell line Jeg 3 and the cDNA was amplified by RT PCR; The cDNA fragment was inserted into the eukaryotic expressing vector pcDNA3 and the recombinant plasmid was identified by restriction endonucleases digestion and sequencing. Results After restriction endonucleases treatment and sequencing, it was confirmed that the pcDNA3 sHLA G1 had been constructed successfully. Conclusion In this study, the recombinant plasmid pcDNA3 sHLA G1 had been constructed successfully.