大肠杆菌表达人幽门螺杆菌尿素酶B亚单位跨膜区成份

Recombination and expression of a component of urease B subunit transmembrane protein of helicobacter pylori

目的重组表达人幽门螺杆菌尿素酶 B亚单位跨膜区成份。方法采用 DNA重组技术将尿素酶 B亚单位 3'端732 bp基因片段克隆至 p ET11C载体上 ,转化宿主菌 BL 2 1(DE3) E.coli,IPTG诱导 ,SDS- PAGE及 Western- blot分析表达情况。结果成功构建了含 Ure B0 .7kb片段的重组质粒 p ET- Ure B0 .7,并表达了具有免疫反应性的分子量约 2 80 0 0 u的重组蛋白 ,表达率为 19.8%。结论重组的尿素酶 B亚单位跨膜区成份为研究其相关生物学特性奠定了重要基础 ,亦可作为 Hp疫苗的成分用于

ObjectiveTo recombine and express a component of urease B subunit transmembrane protein of helicobacter pylori MethodsA 732 bp gene fragment of urease B subunit of helicobacter pylori was cloned into pET11C and transformed into BL21(DE3) E coli The positive clone was induced with IPTG The expression of target protein was analysed by SDS PAGE and Western blot ResultsIt is successful to construct the recombinant plasmid pET UreB0 7 containing urease B subunit 0 7 kb gene fragment A protein (MW≈28 000 u) with immunoreactivity, was expressed by 19 8% in BL21(DE3) E coli induced with IPTG ConclusionsThe recombinant component of urease B subunit transmembrane protein may play a role in the research of its biological function and might be used as the vaccine against helicobacter pylori