大鼠下丘脑室旁核对脾脏免疫功能神经调控的研究

Study of hypothalamic paraventricular nucleus modulating the splenic immune function in the rat—pseudorabies virus transsynaptic labeling

目的探讨 PVN不同亚核在调控脾脏免疫功能中的作用。方法 4只 SD大鼠脾脏内注射假狂犬病毒 (PRV)96 h后 ,采用免疫组化法和体视学方法研究被假狂犬病毒跨突触感染的神经元在 PVN不同亚核内的分布特点及其与 PVN内 AVP神经元位置配布间的相互关系。结果 PRV感染的神经元主要聚集于 PVN尾段的尾侧大细胞亚核 (PM)和外侧小细胞亚核 (L P) (占总面积的 73.49% ) ,少量分布于喙段的前小细胞亚核 (L P)、中段的背侧小细胞亚核 (DP)和室周小细胞亚核内(PP)。其中 L P及 PM内的部分 PRV感染神经元分布在 AVP阳性神经元聚集的区域内。结论 PVN对脾脏免疫功能的调控除了传统神经内分泌途径外 ,还可能存在下列途径 :PM内的 AVP阳性神经元和 L P、DP及 AP内的神经元通过向延髓背侧及脊髓中间外侧柱的投射 。

ObjectiveTo investigate the possible roles of the different subnuclei of hypothalamic paraventricular nucleus (PNV) which may regulate splenic immune function Methods4 SD rats received splenic injection of pseudorabies virus(PRV) 96 hours after injection, a block containing hypothalamus was taken and cut into 40 μm sections with cryostat Immunohistochemical technique and stereology method were used to detect the distributing character of transsynaptic PRV infection neurons in the PVN and the localization relationship between PRV neurons and AVP neurons in the PVN ResultsTranssynaptic PRV infection neurons distributed through out the PVN, but mainly concentrated in the lateral parvocellular (LP) subnucleus of PVN and posterior magnocellular (PM) subnuclei of PVN (occupied 73 49% of total surface area),a few distributed in the anterior, dorsal and periventricular parvocellular (AP,DP and PP)nuclei of PVN,few scatted in the rest of nuclei of PVN In the LP and PM some of the PRV neurons distributed among the AVP neurons ConclusionsBesides the classical neuro immuno endocrine modulation, the PVN may regulate the splenic immune function through direct innervation The AVP neurons in the PM and neurons projecting to the proganglionic neurons in the dorsal medulla and lateral horn of spinal cord in the LP,DP and AP regulate the immune function of spleen through sympathetic and para sympathetic innovation