摘要
目的探讨 bcl- 2、bax在丁型肝炎发病机理中的作用。方法采用免疫组化双标记染色和连续切片技术 ,检测 77例丁型肝炎病人肝组织 HDAg、bcl- 2和 bax表达 ,以 6 7例乙型肝炎作对照。结果 HDAg以肝细胞核表达为主 ,bcl- 2以肝细胞浆及核膜表达为主 ,bax以肝细胞浆表达为主 ,胞核也可呈阳性表达。bax和 HDAg表达及分布有相关性 ,在各临床类型肝炎中的表达强度有显著性差异 (P<0 .0 1)。结论 HDV感染可诱导肝细胞表达 bax,增强 bax促细胞凋亡作用 ,bcl- 2 /
ObjectiveTo study the role of bcl 2 and bax in the pathogenesis of hepatitis D MethodsExpression of HDAg, bcl 2 and bax in liver specimens of 79 patients with hepatitis D were studied by immunohistochemistry technique Meanwhile, the relationship between expression of HDAg and that of bcl 2/bax in liver specimens of patients were studied by double labelling technique and serial sections Resultsbcl 2 was mainly expressed in the cytoplasm of hepatocytes, bax mainly in the cytoplasm of hepatocytes and partly in the nucleus of hepatocytes, and HDAg mainly in the nucleus of hepatocytes Most of HDAg and bax positive cells were distributed among infiltrating lymphocytes at the periportal region especially at the advancing edges of areas of “piecemeal necrosis” Most of hepatocytes of bax positive was found to locate near to positive cells of HDAg, and there were positive correlations between degrees of bax expression and HDAg expression( P <0 05) ConclusionsThe distribution and the expression of bax and HDAg are significantly correlated with the activity of inflammation and the severity of the liver damage, and HDV infection may induce the expression of bax in the hepatocytes,and hepatocyte apoptosis mediated by bcl 2/bax may play an important role in the liver cell injury by HDV infection
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第2期128-131,共4页
Immunological Journal
