人视网膜胶质细胞切线方向收缩的测定

Tangential contraction of human retinal glial cell in vitro

目的 测定细胞离体培养中的人视网膜胶质细胞切线方向收缩。方法 人视网膜胶质细胞提取并用 GFAP和 Vimentin确定细胞属性 ,培养到第二代时 ,将细胞置于胶原凝胶上 ,培养 0～ 2 4h后测定胶原凝胶厚度。结果 人视网膜胶质细胞培养至 6 h后可使胶原凝胶厚度明显减小 ,12 0 0 0细胞培养 2 4h可使凝胶厚度减小 15 % ,而对照组 (无细胞组 )凝胶厚度则无明显改变。结论 本结果提示第二代人视网膜胶质细胞有产生切线方向收缩的能力 ,为

Objective To measure the tangential contraction of human retinal glial cell in vitro .Methods Glial cells were harvested from human retina within 2 hours after death. Cell identities were recognized with GFAP and vimentin localization. Human retinal glial cells were cultured on the surface of collagen gel for 0～24 hours, thickness of the gel were measured at different time.Results Human retinal glial cells could reduce the thickness of collagen gel significantly (up to 15%), while no significant changes occurred among gels of control group (with no cells).Conclusions Human retinal glial cells can produce measurable tangential contraction force. These results provide another evidence for Gass's new mechanism of idiopathic macular hole formation.