GC-MS追踪筛选加味苍耳子散挥发油提取工艺

Follow up Screening of Extraction Process of Volatile Oil in Supplemented Xanthium Powder by GC MS

目的 :筛选加味苍耳子散挥发油提取工艺。方法 :采用GC MS对组方中每味药材所含挥发油的主要成分进行追踪。结果 :确定加味苍耳子散挥发油提取工艺为 :辛夷、丹皮粉碎成粗粉 ,合并薄荷采用水蒸气蒸馏法提油 ,收集 13倍的初蒸馏液 ,再收集 3倍量的重蒸馏液 ;川芎挥发油采用醇提 ;苍耳子、白芷含挥发油很少 ,同时为避免苍耳子水提产生毒性及白芷香豆素类成分遭破坏 ,其油不予提取。结论 :GC MS是追踪优化中药复方挥发油提取工艺的强有力工具 。

Objective: To screen the extraction process of volatile oil in Supplemented Xanthium Powder.Methods: The main compositions of volatile oil contained by every herb in prescription were followed up by GC MS. Results: The extraction process of volatile oil in Supplemented Xanthium Powder was established, that is, Flos Magnoliae and Cortex Moutan Radicis were powdered, their volatile oils were extracted with Herba Menthae together by the steam distillation; then 13 fold first fraction of distillata and 3 fold redistillation liquid were collected respectively; the volatile oil of Rhizoma Liqustici Chuanxiong was extracted with alcohol. Inasmuch as Fructus Xanthii and Radix Angelicae Dahuricae contained less volatile oil, at the same time, to avoide that Fructus Xanthii produced toxicity by water extraction and coumarinic lactones of Radix Angelicae Dahuricae was destroyed their oils were considered unfit for extraction. Conclusion: GC MS is a useful way for follow up optimization of the extraction process of volatile oil in compound prescription of Chinese medicinal and worthy of popularization.