微乳TLC同时分离鉴定2种成药的多种成分

Separation and Identification of Various Effective Compositions in Two kinds of Traditional Chinese Patent Medicines by Microemulsion TLC

目的 :探讨微乳薄层色谱法用于同时分离鉴定中成药中多种有效成分。方法 :以 6种SDS/正丁醇 /正庚烷 /水微乳液为展开剂 ,聚酰胺薄膜为固定相 ,通过一次点样展开 ,分别检测抗感冒颗粒剂和防风通圣丸中多种成分。考察了微乳液类型、酸度等因素对分离效果的影响。结果 :以含水量 75 %的微乳液 甲酸 (9∶1)为展开剂 ,可同时检出抗感冒颗粒剂中黄芩甙、绿原酸、防风和甘草 ,防风通圣丸中大黄素、大黄酚、黄芩甙、防风和甘草 ,操作简便 ,分离效果理想 ,检出灵敏。结论 :微乳薄层色谱法是一种高效简捷的新方法 ,可同时分离鉴定中成药中多种有效成分。

Objective: To investigate the application of microemulsion TLC in separation and identification of various effective compositions in traditional Chinese patent medicines. Methods: The various compositions in Kangganmao Granules and Fangfeng Tongsheng Pills were separated and identified simultaneously on polyamide film with six kinds of SDS/n C 4H 9OH/n C 7H 16 /H 2O microemulsions as the mobile phases. Affections of kinds and acidity on separationg results of microemulsions were investigated. Results: The baicalin, chlorogenic acid, Saposhnikovia and glycyrrhiza in Kangganmao Gronules and the emodin, chrysophanol, baicalin, Saposhnikovia and Glycyrrhizae in Fangfeng Tongsheng Pills may be separated and identified simultaneously with microemulsion (containing 75% water) formic acid (9∶1) as mobile phase. Conclusion: Microemulsion TLC is an efficient, simple new method for the separation and identification of various compositions in traditional Chinese patent medicines.