糖尿病大鼠局灶脑缺血/再灌注损伤与脑组织TGF-β_1 mRNA表达(英文)

Brain damage of cerebral ischemia/reperfusion and expression of TGF-β_1 mRNA in diabetic rats

目的 :观察糖尿病和对照组鼠脑缺血 /再灌注损伤与转化生长因子 β1(TGF - β1)mRNA表达的异质性。方法 :采用逆转录聚合酶链式反应 (RT -PCR)检测大鼠中脑动脉阻塞 (MCAO)后脑内TGF - β1mRNA表达水平 ,并且应用组织病理进行损伤程度评定。结果 :糖尿病组大鼠脑缺血及缺血 /再灌损伤程度明显重于“相应对照组”。糖尿病及对照组大鼠在MCAO 2h时TGF - β1mRNA表达量明显增高 ,后者增高比前者更为明显。再灌 2 4h后TGF -β1mRNA表达量下降 ,但仍高于假手术组。结论 :糖尿病加重缺血 /再灌性脑损伤 ;MCAO后TGF - β1mRNA表达增高可能是机体一种抗损伤反应 。

AIM: To observe the difference of cerebral injury following ischemia/reperfusion and mRNA expression of TGF-β 1 between diabetic and non-diabetic rats.METHODS: At first, Wistar rats were divided into two groups,non-diabetes and diabetes, and then two groups followed by sham, middle cerebral artery occlusion(MCAO) 2h and reperfusion 24 h after MCAO 2 h respectively. TGF-β 1 mRNA expression was measured by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR); Cerebral damage was evaluated by histopathology.RESULTS: In the same condition of ischemia or ischemia/reperfusion, injuried area enlarged in DM groups; The expression level of TGF-β 1 mRNA increased at the time of 2 h after MCAO in non-diabetic group and diabetic group, especialy significantly in non-diabetic group with MCAO 2 h, and decreased at the time of reperfusion 24 h after MCAO 2 h, but still higher than that in the sham group.CONCLUSION: Diabetes mellitus exacerbated brain lesion following ischemia/repefusion, increased TGF-β 1 mRNA expresion after MCAO may be an anti-injury reaction,and anti-injury ability is decreased under diabetic condition.