摘要
目的研究前列腺癌、前列腺增生组织中PRL-3(phosphatase of regenerating liver-3,PRL-3)基因的表达,探讨其与患者临床病理特征的关系。方法分别采用免疫组织化学S-P法和实时荧光定量PCR检测54例前列腺穿刺/手术切除标本中的PRL-3蛋白和mRNA,其中前列腺增生组织20例,前列腺癌组织34例,比较两者间基因表达的差异;研究PRL-3蛋白和mRNA表达水平与前列腺癌患者年龄、Gleason评分、神经侵犯等临床病理特征的关系和相关性。结果PRL-3蛋白和mRNA在前列腺癌及前列腺增生组织中均可检出,癌组织中的PRL-3蛋白和mRNA表达水平显著高于增生组织(P=0.001;P=0.001)。PRL-3蛋白和mRNA表达水平均与前列腺癌患者年龄无关。PRL-3蛋白与前列腺癌神经侵犯和Gleason评分无关(P=0.376;P=0.360)。PRL-3 mRNA与Gleason评分密切相关(P=0.001);PRL-3 mRNA与神经侵犯也存在相关P=0.001。结论 PRL3基因的表达与前列腺癌发生发展机制关系较密切,可作为前列腺癌鉴别诊断及判断预后的一种较有价值的病理学指标。
Objective To investigate the association of PRL-3 protein and mRNA with clinico- pathological characteristics of prostatic carcinoma. Methods Tissue specimens were obtained from 54 patients undergoing needle biopsy or surgery, including 34 cases of prostatic carcinoma and 20 cases of benign prostate hyperplasia. Immunohistochemical staining and real-time quantitative reverse transcriptional PCR（qRT-PCR） were performed, and the differential expression of PRL-3 protein and mRNA were analyzed for their associa tion with clinicopathological characteristics of the prostatic carcinoma patients. Results PRL-3 protein and mRNA were detected in prostatic carcinoma and benign prostate hyperplasia, and PRL-3 protein and mRNA were significantly higher in prostatic carcinoma than that in benign prostate hyperplasia. The PRL-3 expression was highly correlated with Gleason score and perineural invasion, but not with the patient＇s age. Conclusions PRL-3 gene plays an crucial role in prostate carcinogenesis and can be used as a biomarker for the prognosis of patients with prostate cancer.
出处
《分子诊断与治疗杂志》
2014年第3期157-161,共5页
Journal of Molecular Diagnostics and Therapy
