实验动物绿脓杆菌的检测方法研究

Study on Identification Method for Pseudomonas aeruginosa in Laboratory Animals

目的研究和建立实验动物及环境样品绿脓杆菌(Pseudomonas aeruginosa)快速、准确的鉴定方法。方法分别用VITEK2 Compact全自动分析系统和常规生化方法对绿脓杆菌参考菌株、绿脓杆菌分离株和恶臭假单胞菌分离株进行鉴定试验,并比较不同鉴别培养基的鉴定效果。结果可以用PIA琼脂培养基/CFC琼脂培养基和乙酰胺琼脂培养基为鉴定培养基,采用以氧化酶、明胶液化和42℃生长试验为主的常规鉴定方法,也可采用VITEK2Compact全自动微生物分析系统直接鉴定绿脓杆菌或将其用于常规鉴定结果的确认。结论 VITEK2 Compact全自动微生物分析系统适于绿脓杆菌快速、准确的鉴定或用于对常规鉴定结果的确认。

Objective To study and establish accurate and rapid identification methods of Pseudomonas aeruginosa in laboratory animals. Method VITEK2 Compact automatic microbiology system and conventional biochemical methods were used to identify Pseudomonas aeruginosa reference strain, Pseudomonas aeruginosa and Pseudomonas putida isolate strains. The identification results of 4 differential media were also evaluated. Result For conventional identification methods, PIA or CFC agar media and acetamide agar media can be used as differential media, and some biochemical tests such as O/F glucose, oxidase, 42℃ growth test were essential for the identification of Pseudomonas aeruginosa. VITEK2 Compact automated microbiology system enables the differentiation of Pseudomonas aeruginosa from similar bacteria and can serve as additional confirmatory method in conventional biochemical tests. Conclusion VITEK2 Compact automated microbiology system is able to identify Pseudomonas aeruginosa in laboratory animals accurately and efficiently.